Functional analysis of theEscherichia colizinc transporter ZitB
- 1 October 2002
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 215 (2), 273-278
- https://doi.org/10.1111/j.1574-6968.2002.tb11402.x
Abstract
The membrane transporter ZitB responsible for Zn(II) efflux in Escherichia coli was studied by site-directed mutagenesis to elucidate the function of individual amino acid residues. Substitutions of several charged or polar residues, H53, H159, D163 and D186, located in predicted transmembrane domains resulted in loss of ZitB function. In contrast, neither the amino-terminal nor the carboxy-terminal regions, both histidine-rich, were required for function.Keywords
This publication has 15 references indexed in Scilit:
- Escherichia coli CopA N-Terminal Cys(X)2Cys Motifs Are Not Required for Copper Resistance or TransportBiochemical and Biophysical Research Communications, 2001
- The kamikaze approach to membrane transportNature Reviews Molecular Cell Biology, 2001
- ZitB (YbgR), a Member of the Cation Diffusion Facilitator Family, Is an Additional Zinc Transporter in Escherichia coliJournal of Bacteriology, 2001
- NreB from Achromobacter xylosoxidans 31A Is a Nickel-Induced Transporter Conferring Nickel ResistanceJournal of Bacteriology, 2001
- Expression of ZRC1 Coding for Suppressor of Zinc Toxicity Is Induced by Zinc-starvation Stress in Zap1-Dependent Fashion in Saccharomyces cerevisiaeBiochemical and Biophysical Research Communications, 2000
- Functional Analysis of the N-terminal CXXC Metal-binding Motifs in the Human Menkes Copper-transporting P-type ATPase Expressed in Cultured Mammalian CellsPublished by Elsevier BV ,1999
- THE MOLECULAR BIOLOGY OF METAL ION TRANSPORT INSACCHAROMYCES CEREVISIAEAnnual Review of Nutrition, 1998
- A Novel Family of Ubiquitous Heavy Metal Ion Transport ProteinsThe Journal of Membrane Biology, 1997
- Ion efflux systems involved in bacterial metal resistancesJournal of Industrial Microbiology & Biotechnology, 1995
- Site-directed mutagenesis by overlap extension using the polymerase chain reactionGene, 1989