Association of Flavin Adenine Dinucleotide with the Arabidopsis Blue Light Receptor CRY1
- 18 August 1995
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 269 (5226), 968-970
- https://doi.org/10.1126/science.7638620
Abstract
The arabidopsis thaliana HY4 gene encodes CRY1, a 75-kilodalton flavoprotein mediating blue light-dependent regulation of seedling development. CRY1 is demonstrated here to noncovalently bind stoichiometric amounts of flavin adenine dinucleotide (FAD). The redox properties of FAD bound by CRY1 include an unexpected stability of the neutral radical flavosemiquinone (FADH.). The absorption properties of this flavosemiquinone provide a likely explanation for the additional sensitivity exhibited by CRY1-mediated responses in the green region of the visible spectrum. Despite the sequence homology to microbial DNA photolyases, CRY1 was found to have no detectable photolyase activity.Keywords
This publication has 20 references indexed in Scilit:
- Putative Blue-Light Photoreceptors from Arabidopsis thaliana and Sinapis alba with a High Degree of Sequence Homology to DNA Photolyase Contain the Two Photolyase Cofactors but Lack DNA Repair ActivityBiochemistry, 1995
- The Transduction of Blue Light Signals in Higher PlantsAnnual Review of Plant Physiology and Plant Molecular Biology, 1994
- HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptorNature, 1993
- Cloning and Nucleotide Sequence of a Tobacco Chloroplast Translational Elongation Factor, EF-TuPlant Physiology, 1993
- Chromophore function and interaction in Escherichia coli DNA photolyase: reconstitution of the apoenzyme with pterin and/or flavin derivativesBiochemistry, 1990
- Identification of oligothymidylates as new simple substrates for E. coli DNA photolyase and their use in a rapid spectrophotometric enzyme assayBiochemistry, 1985
- Identification of a neutral flavin radical and characterization of a second chromophore in Escherichia coli DNA photolyaseBiochemistry, 1984
- BLUE LIGHT PHOTORECEPTIONPhotochemistry and Photobiology, 1979
- Light-mediated reduction of flavoproteins with flavins as catalystsBiochemistry, 1978
- Riboflavin, Light, and the Growth of PlantsScience, 1950