Characterization of the Cytotoxic Effect of High-Intensity, 10-ns Duration Electrical Pulses
- 8 October 2004
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Transactions on Plasma Science
- Vol. 32 (4), 1579-1586
- https://doi.org/10.1109/tps.2004.831773
Abstract
Cytotoxic effects of 10-ns electrical pulses (EP, 50-380 kV/cm) were analyzed in cultured U937 cells (human lymphoma). Densities of live and dead cells were compared in over 500 samples at intervals from 0.5 to 48 h post exposure. EP trains of 1-20 pulses caused a minor, if any, decrease in cell survival: 24 h post exposure, the density of live cells typically dropped just 10-20% compared with unexposed parallel control. Within studied limits, this effect did not significantly depend on the EP number, voltage, or repetition rate. However, much longer EP trains could cause a sharp survival decline. The transition from plateau to profound cell killing occurred at about 150 pulses at 150 kV/cm, and at over 1000 pulses at 50 kV/cm. Artifact-free thermometry using a fiber optic microprobe established unequivocally that cell killing by extra-long EP trains was not a result of sample heating and has to be explained by other mechanisms. Testing for specific apoptotic cleavage of poly(ADP-ribose) polymerase at scattered time intervals (1-24 h) after EP exposure produced mostly negative results. Overall, 10-ns EP caused far weaker cytotoxic effect than it was reported earlier from experiments in other cell lines and mostly with longer pulses (60 and 300 ns). The survival curve shape (i.e., the shoulder followed by exponential decline) is also characteristic for other cytotoxic factors, such as low-LET ionizing radiation, thereby possibly pointing to common mechanisms or targets.Keywords
This publication has 21 references indexed in Scilit:
- Nanosecond, high‐intensity pulsed electric fields induce apoptosis in human cellsThe FASEB Journal, 2003
- Bioelectrics-new applications for pulsed power technologyIEEE Transactions on Plasma Science, 2002
- Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: apoptosis induction and tumor growth inhibitionIEEE Transactions on Plasma Science, 2002
- Effect of Glutathione Depletion on Antitumor Drug Toxicity (Apoptosis and Necrosis) in U-937 Human Promonocytic CellsPublished by Elsevier BV ,2001
- Heat Shock Protein 70 Inhibits Apoptosis Downstream of Cytochrome c Release and Upstream of Caspase-3 ActivationPublished by Elsevier BV ,2000
- A review of short pulse generator technologyIEEE Transactions on Plasma Science, 2000
- Electroporation of cells and tissuesIEEE Transactions on Plasma Science, 2000
- Distinct cleavage products of nuclear proteins in apoptosis and necrosis revealed by autoantibody probesCell Death & Differentiation, 1998
- Hsp70 Prevents Activation of Stress KinasesPublished by Elsevier BV ,1997
- Auditory perception of radio-frequency electromagnetic fieldsThe Journal of the Acoustical Society of America, 1982