Identification of Novel Avian Influenza Virus Derived CD8+ T-Cell Epitopes
Open Access
- 23 February 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (2), e31953
- https://doi.org/10.1371/journal.pone.0031953
Abstract
Avian influenza virus (AIV) infection is a continuing threat to both humans and poultry. Influenza virus specific CD8+ T cells are associated with protection against homologous and heterologous influenza strains. In contrast to what has been described for humans and mice, knowledge on epitope-specific CD8+ T cells in chickens is limited. Therefore, we set out to identify AIV-specific CD8+ T-cell epitopes. Epitope predictions based on anchor residues resulted in 33 candidate epitopes. MHC I inbred chickens were infected with a low pathogenic AIV strain and sacrificed at 5, 7, 10 and 14 days post infection (dpi). Lymphocytes isolated from lung, spleen and blood were stimulated ex vivo with AIV-specific pooled or individual peptides and the production of IFNγ was determined by ELIspot. This resulted in the identification of 12 MHC B12-restricted, 3 B4-restricted and 1 B19-restricted AIV- specific CD8+ T-cell epitopes. In conclusion, we have identified novel AIV-derived CD8+ T-cell epitopes for several inbred chicken strains. This knowledge can be used to study the role of CD8+ T cells against AIV infection in a natural host for influenza, and may be important for vaccine development.Keywords
This publication has 57 references indexed in Scilit:
- Cross reactive cellular immune responses in chickens previously exposed to low pathogenic avian influenzaBMC Proceedings, 2011
- Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responsesVeterinary Research, 2011
- Non-replicating adenovirus vectors expressing avian influenza virus hemagglutinin and nucleocapsid proteins induce chicken specific effector, memory and effector memory CD8+ T lymphocytesVirology, 2010
- Summary of Avian Influenza Activity in Europe, Asia, and Africa, 2006–2009Avian Diseases, 2010
- Avian Influenza in North and South America, the Caribbean, and Australia, 2006–2008Avian Diseases, 2010
- Attacking the flu: Neutralizing antibodies may lead to 'universal' vaccineNature Medicine, 2009
- Memory T cells established by seasonal human influenza A infection cross-react with avian influenza A (H5N1) in healthy individualsJCI Insight, 2008
- Evolutionarily Conserved Protein Sequences of Influenza A Viruses, Avian and Human, as Vaccine TargetsPLOS ONE, 2007
- Resistance to and Recovery from Lethal Influenza Virus Infection in B Lymphocyte–deficient MiceThe Journal of Experimental Medicine, 1997
- Cytotoxic T-Cell Immunity to InfluenzaNew England Journal of Medicine, 1983