Subendothelial proteins and platelet adhesion. von Willebrand factor and fibronectin, not thrombospondin, are involved in platelet adhesion to extracellular matrix of human vascular endothelial cells.
- 1 January 1986
- journal article
- abstracts
- Published by Ovid Technologies (Wolters Kluwer Health) in Arteriosclerosis: An Official Journal of the American Heart Association, Inc.
- Vol. 6 (1), 24-33
- https://doi.org/10.1161/01.atv.6.1.24
Abstract
Endothelial cell matrix contained von Willebrand factor (VWF), fibronectin, and thrombospondin. The role of these proteins in the adhesion of platelets was investigated by preincubation of the matrix with specific antibodies and subsequent perfusion with human blood. When perfusions were performed with platelets in a human albumin solution (HAS) platelet adhesion was similar to that with normal plasma, indicating that proteins in the matrix can fully support adhesion. Preincubation of the matrix with a monoclonal antibody to VWF and perfusion with HAS showed a nearly complete inhibition of platelet adhesion at 1300 s-1, indicating a role for matrix-bound VWF at high shear rates and no requirement for VWF in plasma. Preincubation of the matrix with antihuman fibronectin F(ab')2 showed a slight inhibition of adhesion. The same result was obtained with perfusions with fibronectin-free plasma, and an untreated matrix. Preincubation with antifibronectin F(ab')2 and perfusion with fibronectin-free plasma showed a significant inhibition of platelet adhesion at all shear rates. These results indicate that fibronectin is required for adhesion at all shear rates. Preincubation of the matrix with different antibodies against human platelet thrombospondin showed no inhibition of platelet adhesion at all wall shear rates. Thrombospondin in the matrix is evidently not required for platelet adhesion.This publication has 25 references indexed in Scilit:
- Role of factor VIII-von Willebrand factor and fibronectin in the interaction of platelets in flowing blood with monomeric and fibrillar human collagen types I and III.JCI Insight, 1985
- Analysis of platelet adhesion with a radioactive chemical crosslinking reagent: Interaction of thrombospondin with fibronectin and collagenCell, 1982
- Thrombospondin: synthesis and secretion by cells in culture.The Journal of cell biology, 1982
- Synthesis and secretion of thrombospondin by cultured human endothelial cells.The Journal of cell biology, 1982
- Human plasma fibronectin promotes the adhesion and spreading of platelets on surfaces coated with fibrillar collagenFEBS Letters, 1981
- Growth Factors and the Extracellular MatrixEndocrine Reviews, 1980
- Human blood platelet adhesion to artery subendothelium is mediated by factor VIII–Von Willebrand factor bound to the subendotheliumNature, 1979
- Synthesis of fibronectin by cultured human endothelial cells.The Journal of Experimental Medicine, 1978
- Synthesis of Antihemophilic Factor Antigen by Cultured Human Endothelial CellsJCI Insight, 1973
- Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIAJCI Insight, 1973