Confinement‐Induced Enhancement of Antigen–Antibody Interactions within Binary Nanopatterns to Achieve Higher Efficiency of On‐Chip Immunosensors
- 17 July 2008
- journal article
- research article
- Published by Wiley in Advanced Materials
- Vol. 20 (14), 2782-2788
- https://doi.org/10.1002/adma.200702188
Abstract
By combining molecular self-assembly, nanosphere lithography and reactive ion etching, large-scale nanopatterns of antibodies are fabricated for direct application in state-of-the-art on-chip immunosensors. Using in-situ surface plasmon resonance, the patterns are studied in view of their antigen binding capacity, which shows an increase of up to 120% solely in the case of antibody confinement into the nanopatches by means of a nonfouling embedding matrix (see figure).Keywords
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