Glycoform Analysis of Japanese Cedar Pollen Allergen, Cry j 1
- 1 January 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Bioscience, Biotechnology, and Biochemistry
- Vol. 69 (9), 1700-1705
- https://doi.org/10.1271/bbb.69.1700
Abstract
In our previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 69, 137-144 (2005)), we found that plant complex type N-glycans harboring Lewis a epitope are linked to the mountain cedar pollen allergen Jun a 1. Jun a 1 is a glycoprotein highly homologous with Japanese cedar pollen glycoallergen, Cry j 1. Although it has been found that some plant complex type N-glycans are linked to Cry j 1, the occurrence of Lewis a epitope in the N-glycan moiety has not been proved yet. Hence, we reinvestigated the glycoform of the pollen allergen to find whether the Lewis a epitope(s) occur in the N-glycan moiety of Cry j 1. From the cedar pollen glycoallergen, the N-glycans were liberated by hydrazinolysis and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC. The structures were analyzed by a combination of exo- and endo-glycosidase digestions, sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS). Structural analysis clearly indicated that Lewis a epitope (Galbeta1-3(Fucalpha1-4)GlcNAcbeta1-), instead of the Galbeta1-4(Fucalpha1-6)GlcNAc, occurs in the N-glycans of Cry j 1.Keywords
This publication has 12 references indexed in Scilit:
- Occurrence of Lewis a Epitope inN-Glycans of a Glycoallergen, Jun a 1, from Mountain Cedar (Juniperus ashei) PollenBioscience, Biotechnology, and Biochemistry, 2005
- Structural Features ofN-Glycans Linked to Glycoproteins from Oil Palm Pollen, an Allergenic Pollen*Bioscience, Biotechnology, and Biochemistry, 2003
- Monitoring Endoplasmic Reticulum-to-Golgi Traffic of a Plant Calreticulin by Protein Glycosylation AnalysisBiochemistry, 2002
- Analysis of Asn-linked glycans from vegetable foodstuffs: widespread occurrence of Lewis a, core 1,3-linked fucose and xylose substitutionsGlycobiology, 2001
- N-Linked Oligosaccharides of Glycoproteins from Ginkgo biloba Pollen, An Allergenic PollenBioscience, Biotechnology, and Biochemistry, 2001
- Molecular cloning of the mountain cedar (Juniperus ashei) pollen major allergen, Jun a 1Journal of Allergy and Clinical Immunology, 1999
- N‐glycans harboring the Lewis a epitope are expressed at the surface of plant cellsThe Plant Journal, 1997
- Structures and contribution to the antigenicity of oligosaccharides of Japanese cedar (Cryptomeria japonica) pollen allergenCry j I: relationship between the structures and antigenic epitopes of plantN-linked complex-type glycansGlycoconjugate Journal, 1996
- N‐terminal amino acid sequence of a major allergen of Japanese cedar pollen (Cry j I)FEBS Letters, 1988
- Structures of Sugar Chains of Ricin DThe Journal of Biochemistry, 1988