Mosaic Qβ coats as a new presentation model
- 10 July 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 431 (1), 7-11
- https://doi.org/10.1016/s0014-5793(98)00716-9
Abstract
The new protein carrier was developed on the basis of recombinant RNA phage Qβ capsid. C-terminal UGA extension of the short form of Qβ coat, so-called A1 extension, served as a target for presentation of foreign peptides on the outer surface of mosaic Qβ particles. In conditions of enhanced UGA suppression, the proportion of A1-extended to short coats in mosaic particles dropped from 48% to 14%, with an increase of the length of A1 extension. A model insertion, short preS1 epitope 31-DPAFR-35 of hepatitis B surface antigen, demonstrated superficial location on the mosaic Qβ particles and ensured specific antigenicity and immunogenicity.Keywords
This publication has 20 references indexed in Scilit:
- The crystal structure of bacteriophage GA and a comparison of bacteriophages belonging to the major groups of Escherichia coli levivirusesJournal of Molecular Biology, 1997
- Phase display of proteinsCurrent Opinion in Biotechnology, 1996
- The crystal structure of bacteriophage Qβ at 3.5 å resolutionStructure, 1996
- Recombinant rna phage qβ capsid particles synthesized and self-assembled in escherichia coliGene, 1993
- Enhanced readthrough of opal (UGA) stop codons and production of Mycoplasma pneumoniae P1 epitopes in Escherichia coliGene, 1993
- Analysis of RNA phage fr coat protein assembly by insertion, deletion and substitution mutagenesisProtein Engineering, Design and Selection, 1993
- Multiple presentation of foreign peptides on the surface of an RNA-free spherical bacteriophage capsidJournal of General Virology, 1993
- Multiple display of foreign peptides on a filamentous bacteriophageJournal of Molecular Biology, 1991
- The readthrough protein A1 is essential for the formation of viable Qβ particlesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- A single UGA codon functions as a natural termination signal in the coliphage Qβ coat protein cistronJournal of Molecular Biology, 1973