Rapid Diagnosis of Bacterial Meningitis by a Seminested PCR Strategy
- 1 January 1995
- journal article
- Published by Informa UK Limited in Scandinavian Journal of Infectious Diseases
- Vol. 27 (5), 537-539
- https://doi.org/10.3109/00365549509047063
Abstract
A polymerase chain reaction (PCR) based diagnostic assay has been developed for the simultaneous detection in cerebrospinal fluid (CSF) of Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, Streptococcus agalactiae, Listeria monocytogenes and bacteria in general. In the present communication we describe the design of primers for S. pneumoniae, S. agalactiae, and L. monocytogenes, and a general PCR protocol for the assay. The diagnostic outcome is presented for a small collection of CSF specimens including 2 samples from patients with culture-negative purulent meningitis.Keywords
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