Adaptation of the Bergman and Loxley technique for hydroxyproline determination to the AutoAnalyzer and its use in determining plasma hydroxyproline in the domestic fowl

Abstract

The adaptation of a manual technique for hydroxyproline determination to the AutoAnalyzer is described. The method can be used to determine standard solutions to within 2·6 per cent. and to deal with successive concentration differences of up to 10 µg ml^–1. The concentration range for optimum accuracy is 2·5 to 15·0 µg ml^–1. Application of the technique to the study of plasma hydroxyproline levels in the domestic fowl is described, and comparison with isotope-dilution analysis demonstrates the lack of gross interference.