Preparation of Thermoresponsive Cationic Copolymer Brush Surfaces and Application of the Surface to Separation of Biomolecules
- 21 March 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 9 (4), 1340-1347
- https://doi.org/10.1021/bm701427m
Abstract
We have prepared poly(N-isopropylacrylamide (IPAAm)-co-2-(dimethylamino)ethylmethacrylate (DMAEMA)) brush-grafted silica bead surfaces through surface-initiated atom transfer radical polymerization (ATRP) using the CuCl/CuCl2/Me6TREN catalytic system in 2-propanol at 25 °C for 16 h. The prepared temperature-responsive surfaces were characterized by chromatographic analysis using the modified silica beads as stationary phases. Chromatographic retention times for adenosine nucleotides in aqueous mobile phases were significantly increased compared to that previously reported for other cationic hydrogel surfaces, indicating that strong electrostatic cationic copolymer brush interactions occur between the surfaces and nucleotide analytes. Retention times for adenosine nucleotides significantly decreased with increasing column temperature, explained by the decreasing basicity in the copolymer with increasing temperature. Step-temperature gradients from 10 to 50 °C shorten ATP retention times. These results indicate that cationic copolymer brush surfaces prepared by ATRP can rapidly alter their electrostatic properties by changing aqueous temperature.Keywords
This publication has 35 references indexed in Scilit:
- Polymer Terminal Group Effects on Properties of Thermoresponsive Polymeric Micelles with Controlled Outer-Shell Chain LengthsBiomacromolecules, 2005
- Cell sheet engineeringMaterials Today, 2004
- Temperature-Responsive Cell Culture Surfaces Enable “On−Off” Affinity Control between Cell Integrins and RGDS LigandsBiomacromolecules, 2004
- Flow Control Valves for Analytical Microfluidic Chips without Mechanical Parts Based on Thermally Responsive Monolithic PolymersAnalytical Chemistry, 2003
- Gene expression control by temperature with thermo-responsive polymeric gene carriersJournal of Controlled Release, 2000
- Thermo-responsive drug delivery from polymeric micelles constructed using block copolymers of poly(N-isopropylacrylamide) and poly(butylmethacrylate)Journal of Controlled Release, 1999
- Thermo-responsive polymer nanoparticles with a core-shell micelle structure as site-specific drug carriersJournal of Controlled Release, 1997
- Site-Specific Conjugation of a Temperature-Sensitive Polymer to a Genetically Engineered ProteinBioconjugate Chemistry, 1994
- Thermo‐responsive polymeric surfaces; control of attachment and detachment of cultured cellsDie Makromolekulare Chemie, Rapid Communications, 1990
- Solution Properties of Poly(N-isopropylacrylamide)Journal of Macromolecular Science: Part A - Chemistry, 1968