Ovarian granulosa cell lines
- 7 August 2004
- journal article
- review article
- Published by Elsevier BV in Molecular and Cellular Endocrinology
- Vol. 228 (1-2), 67-78
- https://doi.org/10.1016/j.mce.2004.04.018
Abstract
The ovary is a complex endocrine gland responsible for production of sex steroids and is the source of fertilizable ova for reproduction. It also produces various growth factors, transcription factors and cytokines that assist in the complex signaling pathways of folliculogenesis. The ovary possesses two primary steroidogenic cell types. The theca cells (and to a lesser extent, the stroma) are responsible for androgen synthesis, and the granulosa cells are responsible for conversion of androgens to estrogens, as well as progesterone synthesis. These cells undergo a transformation in the luteal phase of the menstrual cycle, converting them from estrogen producing, to predominantly progesterone producing cells. Understanding the molecular mechanisms regulating these cells is essential in understanding the regulation of steroidogenesis and reproduction. Creation of appropriate in vitro cell model systems can provide important tools for the study of ovarian function. This has led to the development of ovarian steroidogenic cell lines in several laboratories. Developing theca cell lines has met with limited success. Conversely, numerous human and animal granulosa cell lines have been developed. This review will discuss the existing granulosa cell lines and their characteristics.Keywords
This publication has 65 references indexed in Scilit:
- Murine granulosa cell morphology and function are regulated by a synthetic Arg–Gly–Asp matrixMolecular and Cellular Endocrinology, 2003
- Follicle-Stimulating Hormone Induces Terminal Differentiation in a Predifferentiated Rat Granulosa Cell Line (ROG)Endocrinology, 1997
- Human ovarian tumor cells: a potential model for thecal cell steroidogenesisJournal of Clinical Endocrinology & Metabolism, 1996
- Keratin expression and steroidogenesis in rat granulosa cells, transformed with the Kirsten-ras and SV40 oncogenes singly and in combinationMolecular and Cellular Endocrinology, 1995
- IGF-binding proteins are differentially regulated in an ovarian granulosa cell lineMolecular and Cellular Endocrinology, 1994
- Secretion of Steroids, Growth Factors, and Cytokines by Immortalized Mouse Granulosa Cell Lines1Biology of Reproduction, 1994
- Adenosine 3',5'-monophosphate suppresses metastatic spread in nude mice of steroidogenic rat granulosa cells transformed by simian virus-40 and Ha-ras oncogeneEndocrinology, 1992
- Hormonal regulation of the growth and steroidogenic function of human granulosa cellsJournal of Clinical Endocrinology & Metabolism, 1992
- Physiologic Characterization of Transformed and Cloned Rat Granulosa Cells1Biology of Reproduction, 1989
- Comparative effects of insulin and insulin-like growth factors on DNA synthesis and differentiation of porcine granulosa cellsBiochemical and Biophysical Research Communications, 1984