Phenolic compounds of Hibiscus sabdariffa and influence of organic residues on its antioxidant and antitumoral properties
Open Access
- 1 March 2015
- journal article
- research article
- Published by FapUNIFESP (SciELO) in Brazilian Journal of Biology
- Vol. 75 (1), 69-76
- https://doi.org/10.1590/1519-6984.07413
Abstract
The aim of this study was to evaluate the phenolic and flavonoids contents and the antioxidant and antitumoral activity of leaf and calyx methanolic extracts from Hibiscus sabdariffa (roselle) cultivated with poultry litter and organosuper® under three modes of application. The total phenolic content in the each extract was determined using the Folin-Ciocalteu reagent and for aluminium chloride flavonoids. The antioxidant parameters were analyzed using a 2, 2-diphenyl-1-picrylhydrazyl (DPPH.) free radical scavenging assay. An antitumor colorimetric assay using sulforhodamine B. The highest contents of phenolic and flavonoids were observed in leaf extracts (389.98 and 104.52 mg g–1, respectively) and calyx extracts (474.09 and 148.35 mg g–1, respectively) from plants cultivated with organosuper®, although these values did not differ significantly from those observed for the other treatments. The average IC50 of leaves (43.48 μg mL–1) and calyces (37.15 μg mL–1) demonstrated that both have substances that may contribute to free radical scavenging action. The methanol extract from calyces showed significant selective activity against a leukemia line (K-562), with IC50 values of 0.12 mg mL–1 (organosuper®) and 1.16 mg mL–1 (poultry litter), with concentration-dependent, cytotoxic and cytocidal effects.This publication has 33 references indexed in Scilit:
- Yield, Boll Distribution and Fibre Quality of Hybrid Cotton (Gossypium hirsutum L.) as influenced by Organic and Modern Methods of CultivationJournal of Agronomy and Crop Science, 2006
- The chemical composition and membrane stability activity of some herbs used in local therapy for anemiaJournal of Ethnopharmacology, 2005
- Hibiscus anthocyanins rich extract-induced apoptotic cell death in human promyelocytic leukemia cellsToxicology and Applied Pharmacology, 2005
- Antioxidant activity of the extracts from Dillenia indica fruitsFood Chemistry, 2005
- Phytochemical, pharmacological and toxicological aspects ofHibiscus sabdariffa L.: a reviewPhytotherapy Research, 2005
- Inhibitory effects of Hibiscus sabdariffa L extract on low‐density lipoprotein oxidation and anti‐hyperlipidemia in fructose‐fed and cholesterol‐fed ratsJournal of the Science of Food and Agriculture, 2004
- Hibiscus sabdariffa Extract Inhibits the Development of Atherosclerosis in Cholesterol-Fed RabbitsJournal of Agricultural and Food Chemistry, 2003
- Anthocyanin-Based Natural Colorants: A New Source of Antiradical Activity for FoodstuffJournal of Agricultural and Food Chemistry, 2000
- Oxidants, antioxidants, and the degenerative diseases of aging.Proceedings of the National Academy of Sciences of the United States of America, 1993
- Antioxidant Determinations by the Use of a Stable Free RadicalNature, 1958