Diallyl Disulfide and Diallyl Trisulfide Up-Regulate the Expression of the π Class of Glutathione S-Transferase via an AP-1-Dependent Pathway
- 6 January 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 55 (3), 1019-1026
- https://doi.org/10.1021/jf061874t
Abstract
Garlic organosulfur compounds are recognized as potential chemopreventive compounds. This protection is related to the induction of phase II detoxification enzymes. We previously reported that diallyl disulfide (DADS) and diallyl trisulfide (DATS) up-regulate the gene expression of the π class of glutathione S-transferase (GSTP) and that an enhancer element named GPE I is required for this induction. In the present study, we further investigated the signal pathway involved in DADS and DATS up-regulation of this detoxification enzyme in Clone 9 cells. Cells were cultured with 25−200 μmol/L of DADS or DATS for 24 h. Western and Northern blots showed that both garlic allyl sulfides concentration dependently induced GSTP protein and mRNA expression, respectively. Changes in GST activity toward ethacrynic acid were consistent with the increase in GSTP expression (P < 0.05). Electromobility gel shift assay showed that the DNA binding activity of nuclear activator protein-1 (AP-1) is concentration-dependently increased in the presence of DADS and DATS as compared with that of the control cells. The phosphorylation of c-Jun NH2-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK), but not of p38, was stimulated in the presence of both garlic allyl sulfides. Pretreatment with SP600125 and PD98059, which are JNK and ERK inhibitors, respectively, abolished the increase in AP-1-DNA binding activity and also the induction of GSTP protein by either allyl sulfide. Our results indicate that the effectiveness of DADS and DATS on GSTP expression is likely related to the JNK-AP-1 and ERK-AP-1 signaling pathways and, thus, that DADS and DATS enhance the binding of AP-1 to GPE I. Keywords: Garlic organosulfur compounds; π class of glutathione S-transferase; gene expression; mitogen-activated protein kinase; activator protein-1This publication has 36 references indexed in Scilit:
- p38 MAPK is involved in CB2 receptor‐induced apoptosis of human leukaemia cellsFEBS Letters, 2005
- Diallyl trisulfide-induced apoptosis in human prostate cancer cells involves c-Jun N-terminal kinase and extracellular-signal regulated kinase-mediated phosphorylation of Bcl-2Oncogene, 2004
- Homocysteine and methionine metabolism in ESRD: A stable isotope studyKidney International, 1999
- Temporal Effects of the Detoxification Enzyme Inducer, Benzyl Isothiocyanate: Activation of c-Jun N-terminal Kinase Prior to the Transcription Factors AP-1 and NFκBBiochemical and Biophysical Research Communications, 1999
- Depression of liver microsomal glucose 6-Phosphatase activity in carbon tetrachloride-poisoned rats. Potential synergistic effects of lipid peroxidation and of covalent binding of haloalkane-derived free radicals to cellular components in the processFree Radical Biology & Medicine, 1996
- Activation of Mitogen-activated Protein Kinase by H2O2Published by Elsevier BV ,1996
- The stress-activated protein kinase subfamily of c-Jun kinasesNature, 1994
- Glutathione Transferases and CancerCritical Reviews in Biochemistry and Molecular Biology, 1992
- Effects of pH adjustment and heat treatment on the stability and the formation of volatile compounds of garlicJournal of Agricultural and Food Chemistry, 1989
- Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factorCell, 1987