Spontaneous production of an interleukin 1-like factor by cloned rheumatoid synovial cells in long-term culture.
Open Access
- 1 September 1987
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 80 (3), 786-796
- https://doi.org/10.1172/JCI113135
Abstract
We have cloned adherent synovial cells from rheumatoid synovitis. These can be generally divided into three types, including cells that have the characteristic features of dendritic cells (DCs), macrophagelike cells (MCs) and fibroblastlike cells (FCs), as classified by morphology and immunofluorescent staining. The cloned cells were able to divide and were cultured for up to 11 mo without any significant morphological changes. All the cloned cells were HLA-DR+ after gamma-interferon treatment. Spontaneous production of a factor with interleukin 1 activity by the cloned cells was detected even after long-term culture (the ability, on a per cell basis, being in the following order: DC greater than MC greater than FC). These synovial cells may be important for bony destruction in rheumatoid joints.This publication has 19 references indexed in Scilit:
- Human recombinant interleukin 1 stimulates collagenase and prostaglandin E2 production by human synovial cells.JCI Insight, 1986
- Rheumatoid Synovial Dendritic Cells as Stimulators in Allogeneic and Autologous Mixed Leukocyte Reactions-Comparison with Autologous Monocytes as Stimulator CellsScandinavian Journal of Immunology, 1986
- Identification of immunostimulatory dendritic cells in the synovial effusions of patients with rheumatoid arthritis.JCI Insight, 1985
- Influences of gamma interferon on synovial fibroblast-like cells. Ia induction and inhibition of collagen synthesis.JCI Insight, 1985
- Characterization of the killer cell generated in the autologous mixed leukocyte reaction.The Journal of Experimental Medicine, 1983
- Identification of Three Major Synovial Lining Cell Populations by Monoclonal Antibodies Directed to Ia Antigens and Antigens Associated with Monocytes/Macrophages and FibroblastsScandinavian Journal of Immunology, 1983
- Adenosine phosphorylase-mediated nucleoside toxicityExperimental Cell Research, 1982
- RHEUMATOID ARTHRITIS: A DISEASE OF T-LYMPHOCYTE/MACROPHAGE IMMUNOREGULATIONThe Lancet, 1981
- Some in-vitro comparisons of synovial cells dispersed by trypsin from rheumatoid and nonrheumatoid synovium.Annals Of The Rheumatic Diseases, 1981
- Purification of a factor from human blood monocyte‐macrophages which stimulates the production of collagenase and prostaglandin E2 by cells cultured from rheumatoid synovial tissuesFEBS Letters, 1981