Matrix Metalloproteinases Regulate the Formation of Dendritic Spine Head Protrusions during Chemically Induced Long-Term Potentiation
Open Access
- 16 May 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (5), e63314
- https://doi.org/10.1371/journal.pone.0063314
Abstract
Dendritic spines are are small membranous protrusions that extend from neuronal dendrites and harbor the majority of excitatory synapses. Increasing evidence has shown that matrix metalloproteinases (MMPs), a family of extracellularly acting and Zn2+-dependent endopeptidases, are able to rapidly modulate dendritic spine morphology. Spine head protrusions (SHPs) are filopodia-like processes that extend from the dendritic spine head, representing a form of postsynaptic structural remodeling in response to altered neuronal activity. Herein, we show that chemically induced long-term potentiation (cLTP) in dissociated hippocampal cultures upregulates MMP-9 activity that controls the formation of SHPs. Blocking of MMPs activity or microtubule dynamics abolishes the emergence of SHPs. In addition, autoactive recombinant MMP-9, promotes the formation of SHPs in organotypic hippocampal slices. Furthermore, spines with SHPs gained postsynaptic α-amino-3-hydroxyl-5-methyl-4-isoxazole propionic acid (AMPA) receptors upon cLTP and the synaptic delivery of AMPA receptors was controlled by MMPs. The present results strongly imply that MMP-9 is functionally involved in the formation of SHPs and the control of postsynaptic receptor distribution upon cLTP.Keywords
This publication has 52 references indexed in Scilit:
- Activation of NMDA receptors promotes dendritic spine development through MMP-mediated ICAM-5 cleavageThe Journal of cell biology, 2007
- Matrix metalloproteinase‐7 disrupts dendritic spines in hippocampal neurons through NMDA receptor activationJournal of Neurochemistry, 2006
- Regulation of matrix biology by matrix metalloproteinasesCurrent Opinion in Cell Biology, 2004
- Eph/ephrin signaling in morphogenesis, neural development and plasticityCurrent Opinion in Cell Biology, 2004
- Eph Receptors, Ephrins, and Synaptic FunctionThe Neuroscientist, 2004
- Forskolin-Induced LTP in the CA1 Hippocampal Region Is NMDA Receptor DependentJournal of Neurophysiology, 2004
- Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signalingNature Neuroscience, 2002
- Chapter 9 Changing views of Cajal's neuron: the case of the dendritic spineProgress in Brain Research, 2002
- How Matrix Metalloproteinases Regulate Cell BehaviorAnnual Review of Cell and Developmental Biology, 2001
- The small pyramidal neuron of the rat cerebral cortex. The perikaryon, dendrites and spinesJournal of Anatomy, 1970