Transposition of an antibiotic resistance element in mycobacteria
- 1 June 1990
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 345 (6277), 739-743
- https://doi.org/10.1038/345739a0
Abstract
Bacterial resistance to antibiotics is often plasmid-mediated and the associated resistance genes encoded by transposable elements. Mycobacteria, including the human pathogens Mycobacterium tuberculosis and M. leprae, are resistant to many antibiotics, and their cell-surface structure is believed to be largely responsible for the wide range of resistance phenotypes. Antibiotic-resistance plasmids have so far not been implicated in resistance of mycobacteria to antibiotics. Nevertheless, antibiotic-modifying activities such as aminoglycoside acetyltransferases and phosphotransferases have been detected in fast-growing species. beta-lactamases have also been found in most fast- and slow-growing mycobacteria. To date no mycobacterial antibiotic-resistance genes have been isolated and characterized. We now report the isolation, cloning and sequencing of a genetic region responsible for resistance to sulphonamides in M. fortuitum. This region also contains an open reading frame homologous to one present in Tn1696 (member of the Tn21 family) which encodes a site-specific integrase. The mycobacterial resistance element is flanked by repeated sequences of 880 base pairs similar to the insertion elements of the IS6 family found in Gram+ and Gram- bacteria. The insertion element is shown to transpose to different sites in the chromosome of a related fast-growing species, M. smegmatis. The characterization of this element should permit transposon mutagenesis in the analysis of mycobacterial virulence and related problems.Keywords
This publication has 16 references indexed in Scilit:
- On the evolution of Tn21-like multiresistance transposons: Sequence analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-I), another member of the Tn21-based expression cassetteMolecular Genetics and Genomics, 1989
- Nucleotide sequence and characterization of a new insertion element, IS240, from Bacillus thuringiensis israelensisPlasmid, 1989
- Complete nucleotide sequence of pAL5OOO, a plasmid from Mycobacterium fortuitumGene, 1988
- Site-specific recombination promotes linkage between trimethoprim- and sulfonamide resistance genes. Sequence characterization of dhfrV and sulI and a recombination active locus of Tn21Molecular Genetics and Genomics, 1988
- Transposon Tn21 encodes a RecA-independent site-specific integration systemMolecular Genetics and Genomics, 1988
- IS431, a staphylococcal insertion sequence-like element related to IS26 from Proteus vulgarisGene, 1987
- Patterns and Distribution of Aminoglycoside-Acetylating Enzymes in Rapidly Growing MycobacteriaAmerican Review of Respiratory Disease, 1987
- Introduction of foreign DNA into mycobacteria using a shuttle phasmidNature, 1987
- Nucleotide sequence of the transposable element IS15Gene, 1984
- Nucleotide sequence of IS26, a new prokaryotic mobile genetic etementNucleic Acids Research, 1983