Long‐chain alkane production by the yeast Saccharomyces cerevisiae
- 29 December 2014
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 112 (6), 1275-1279
- https://doi.org/10.1002/bit.25522
Abstract
In the past decade industrial-scale production of renewable transportation biofuels has been developed as an alternative to fossil fuels, with ethanol as the most prominent biofuel and yeast as the production organism of choice. However, ethanol is a less efficient substitute fuel for heavy-duty and maritime transportation as well as aviation due to its low energy density. Therefore, new types of biofuels, such as alkanes, are being developed that can be used as drop-in fuels and can substitute gasoline, diesel, and kerosene. Here, we describe for the first time the heterologous biosynthesis of long-chain alkanes by the yeast Saccharomyces cerevisiae. We show that elimination of the hexadecenal dehydrogenase Hfd1 and expression of a redox system are essential for alkane biosynthesis in yeast. Deletion of HFD1 together with expression of an alkane biosynthesis pathway resulted in the production of the alkanes tridecane, pentadecane, and heptadecane. Our study provides a proof of principle for producing long-chain alkanes in the industrial workhorse S. cerevisiae, which was so far limited to bacteria. We anticipate that these findings will be a key factor for further yeast engineering to enable industrial production of alkane based drop-in biofuels, which can allow the biofuel industry to diversify beyond bioethanol. Biotechnol. Bioeng. 2015;112: 1275–1279.Funding Information
- European Research Council (247013)
- Swedish Research Council Formas
This publication has 22 references indexed in Scilit:
- Microbial production of short-chain alkanesNature, 2013
- Advanced biofuel production by the yeast Saccharomyces cerevisiaeCurrent Opinion in Chemical Biology, 2013
- Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coliProceedings of the National Academy of Sciences of the United States of America, 2013
- Fatty Aldehydes in Cyanobacteria Are a Metabolically Flexible Precursor for a Diversity of Biofuel ProductsPLOS ONE, 2013
- Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commoditiesProceedings of the National Academy of Sciences of the United States of America, 2012
- Reconstitution of Plant Alkane Biosynthesis in Yeast Demonstrates That Arabidopsis ECERIFERUM1 and ECERIFERUM3 Are Core Components of a Very-Long-Chain Alkane Synthesis ComplexTHE PLANT CELL ONLINE, 2012
- Introduction: Next generation biofuelsNature, 2011
- Mitochondrial fatty acid synthesis – An adopted set of enzymes making a pathway of major importance for the cellular metabolismProgress in Lipid Research, 2010
- PCR‐mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiaeYeast, 2006
- Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol methodMethods in Enzymology, 2002