A ROCK inhibitor permits survival of dissociated human embryonic stem cells
Top Cited Papers
- 27 May 2007
- journal article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 25 (6), 681-686
- https://doi.org/10.1038/nbt1310
Abstract
Poor survival of human embryonic stem (hES) cells after cell dissociation is an obstacle to research, hindering manipulations such as subcloning. Here we show that application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency (from approximately 1% to approximately 27%) and facilitates subcloning after gene transfer. Furthermore, dissociated hES cells treated with Y-27632 are protected from apoptosis even in serum-free suspension (SFEB) culture and form floating aggregates. We demonstrate that the protective ability of Y-27632 enables SFEB-cultured hES cells to survive and differentiate into Bf1(+) cortical and basal telencephalic progenitors, as do SFEB-cultured mouse ES cells.Keywords
This publication has 29 references indexed in Scilit:
- Efficient generation of retinal progenitor cells from human embryonic stem cellsProceedings of the National Academy of Sciences of the United States of America, 2006
- Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passageBiochemical and Biophysical Research Communications, 2006
- Stem cells for the treatment of neurological disordersNature, 2006
- Neural conversion of ES cells by an inductive activity on human amniotic membrane matrixProceedings of the National Academy of Sciences of the United States of America, 2006
- Neurotrophins mediate human embryonic stem cell survivalNature Biotechnology, 2006
- Generation of Tyrosine Hydroxylase Positive Neurons from Human Embryonic Stem Cells after Coculture with Cellular Substrates and Exposure to GDNFThe International Journal of Cell Cloning, 2004
- Directed Differentiation of Embryonic Stem Cells into Motor NeuronsCell, 2002
- Anoikis mechanismsCurrent Opinion in Cell Biology, 2001
- Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cellsNature Biotechnology, 2000
- Embryonic Stem Cell Lines Derived from Human BlastocystsScience, 1998