Highly Pathogenic Avian Influenza Virus Subtype H5N1 Escaping Neutralization: More than HA Variation
- 1 February 2012
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 86 (3), 1394-1404
- https://doi.org/10.1128/jvi.00797-11
Abstract
Influenza A viruses are one of the major threats in modern health care. Novel viruses arise due to antigenic drift and antigenic shift, leading to escape from the immune system and resulting in a serious problem for disease control. In order to investigate the escape process and to enable predictions of escape, we serially passaged influenza A H5N1 virus in vitro 100 times under immune pressure. The generated escape viruses were characterized phenotypically and in detail by full-genome deep sequencing. Mutations already found in natural isolates were detected, evidencing the in vivo relevance of the in vitro -induced amino acid substitutions. Additionally, several novel alterations were triggered. Altogether, the results imply that our in vitro system is suitable to study influenza A virus evolution and that it might even be possible to predict antigenic changes of influenza A viruses circulating in vaccinated populations.Keywords
This publication has 47 references indexed in Scilit:
- Virulence of H5N1 virus in mice attenuates after in vitro serial passagesVirology Journal, 2011
- Vaccination with a synthetic peptide from the influenza virus hemagglutinin provides protection against distinct viral subtypesProceedings of the National Academy of Sciences of the United States of America, 2010
- Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccineJCI Insight, 2010
- Contributions of the Avian Influenza Virus HA, NA, and M2 Surface Proteins to the Induction of Neutralizing Antibodies and Protective ImmunityJournal of Virology, 2010
- Hemagglutinin Receptor Binding Avidity Drives Influenza A Virus Antigenic DriftScience, 2009
- Simple, Sensitive, and Swift Sequencing of Complete H5N1 Avian Influenza Virus GenomesJournal of Clinical Microbiology, 2009
- Avian influenza: genetic evolution under vaccination pressureVirology Journal, 2008
- Epitope Mapping of the Hemagglutinin Molecule of a Highly Pathogenic H5N1 Influenza Virus by Using Monoclonal AntibodiesJournal of Virology, 2007
- BioHealthBase: informatics support in the elucidation of influenza virus host–pathogen interactions and virulenceNucleic Acids Research, 2007
- H5 avian and H9 swine influenza virus haemagglutinin structures: possible origin of influenza subtypesThe EMBO Journal, 2002