Maturation Proteins and Sugars in Desiccation Tolerance of Developing Soybean Seeds
Open Access
- 1 September 1992
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 100 (1), 225-230
- https://doi.org/10.1104/pp.100.1.225
Abstract
The desiccation-tolerant state in seeds is associated with high levels of certain sugars and maturation proteins. The aim of this work was to evaluate the contributions of these components to desiccation tolerance in soybean (Glycine max [L.] Merrill cv Chippewa 64). When axes of immature seeds (34 d after flowering) were excised and gradually dried (6 d), desiccation tolerance was induced. By contrast, seeds held at high relative humidity for the same period were destroyed by desiccation. Maturation proteins rapidly accumulated in the axes whether the seeds were slowly dried or maintained at high relative humidity. During slow drying, sucrose content increased to five times the level present in the axes of seeds held at high relative humidity (128 versus 25 μg/axis, respectively). Stachyose content increased dramatically from barely detectable levels upon excision to 483 μg/axis during slow drying but did not increase significantly when seeds were incubated at high relative humidity. Galactinol was the only saccharide that accumulated to higher levels in axes from seeds incubated at high relative humidity relative to axes from seeds that were slowly dried. This suggests that slow drying serves to induce the accumulation of the raffinose series sugars at a point after galactinol biosynthesis. We conclude that stachyose plays an important role in conferring desiccation tolerance.Keywords
This publication has 15 references indexed in Scilit:
- Trehalose in yeast, stress protectant rather than reserve carbohydrateAntonie van Leeuwenhoek, 1990
- Developing Seeds of Ricinus communis L., When Detached and Maintained in an Atmosphere of High Relative Humidity, Switch to a Germinative Mode without the Requirement for Complete DesiccationPlant Physiology, 1989
- The Glassy State in Corn EmbryosPlant Physiology, 1989
- Soybean Seed Water Relations during in Situ and in Vitro Growth and MaturationPlant Physiology, 1989
- Sugars and Desiccation Tolerance in SeedsPlant Physiology, 1988
- Protein Synthesis during Natural and Precocious Soybean Seed (Glycine max [L.] Merr.) MaturationPlant Physiology, 1988
- Lipid-Sugar InteractionsPlant Physiology, 1988
- Galactinol Synthase Activity and Soluble Sugars in Developing Seeds of Four Soybean GenotypesPlant Physiology, 1987
- Cellular responses to extreme water loss: The water-replacement hypothesisCryobiology, 1982
- Abscisic Acid Levels in Soybean Reproductive Structures during DevelopmentPlant Physiology, 1976