Interaction of Cationic Lipid Vesicles with Model Cell MembranesAs Determined by Neutron Reflectivity
- 22 July 2005
- journal article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 21 (17), 7912-7920
- https://doi.org/10.1021/la050957l
Abstract
Transfection of cells by DNA (for the purposes of gene therapy) can be effectively engineered through the use of cationic lipid/DNA “lipoplexes”, although the transfection efficiency of these lipoplexes is sensitive to the neutral “helper” lipid included. Here, neutron reflectivity has been used to investigate the role of the helper lipid present during the interaction of cationic lipid vesicles with model cell membranes. Dimethyldioctadecylammonium bromide (DDAB) vesicles were formed with two different helper lipids, 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) and cholesterol, and the interaction of these vesicles with a supported phospholipid bilayer was determined. DOPE-containing vesicles were found to interact faster with the membrane than those containing cholesterol, and vesicles containing either of the neutral helper lipids were found to interact faster than when DDAB alone was present. The interaction between the vesicles and the membrane was characterized by an exchange of lipid between the membrane and the lipid aggregates in solution; the deposition of vesicle bilayers on the surface of the membrane was not apparent.Keywords
This publication has 12 references indexed in Scilit:
- Small-Angle Neutron-Scattering Studies of Nonionic Surfactant VesiclesThe Journal of Physical Chemistry B, 2000
- Adsorbed and free lipid bilayers at the solid-liquid interfaceZeitschrift für Physik B Condensed Matter, 1999
- Non-Viral Peptide-Based Approaches to Gene DeliveryJournal of Drug Targeting, 1999
- Ultrastructural characterization of cationic liposome-DNA complexes showing enhanced stability in serum and high transfection activity in vivoBiochimica et Biophysica Acta (BBA) - Biomembranes, 1998
- Adsorption of Cationic Surfactants on Silica. Comparison of Experiment and TheoryLangmuir, 1997
- Structure of Monolayers of Tetraethylene Glycol Monododecyl Ether Adsorbed on Self-Assembled Monolayers on Silicon: A Neutron Reflectivity StudyLangmuir, 1996
- The application of the specular reflection of neutrons to the study of surfaces and interfacesJournal of Physics: Condensed Matter, 1990
- Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.Proceedings of the National Academy of Sciences of the United States of America, 1987
- UV/ozone cleaning of surfacesJournal of Vacuum Science & Technology A, 1985
- Supported phospholipid bilayersBiophysical Journal, 1985