A Geminivirus-Satellite Complex is Associated with Leaf Deformity of Mentha (Mint) Plants in Punjab
- 1 October 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Indian Journal of Virology
- Vol. 21 (2), 103-109
- https://doi.org/10.1007/s13337-010-0018-8
Abstract
A widespread leaf deformity disease of mentha (mint), accompanied by whiteflies, the vectors of begomoviruses, was observed in Punjab in the last few years. The presence of begomovirus was indicated by DNA dot-blot analysis using the conserved coat protein and replication-associated protein genes of another begomovirus, Sri Lankan cassava mosaic virus (SLCMV). A DNA fragment (2.0 kb), representing a partial genomic DNA of a begomovirus, amplified from the symptomatic mentha leaves was used to design end-primers and further amplify an additional 0.9 kb fragment, representing the remaining portion of the resident viral DNA. The two sequences, assembled together (2.7 kb), showed that they represented the complete sequence of an isolate of Tomato leaf curl Karnataka virus (ToLCKV) DNA. Using universal betasatellite primers, a 1.4 kb fragment was amplified from the same sample. This cloned DNA fragment showed complete sequence identity with the previously reported Cotton leaf curl Multan betasatellite (CLCuMB). Majority of the symptomatic mentha leaf samples, collected from four districts of Punjab, showed cross-hybridization in DNA dot-blot using cloned SLCMV and CLCuMB DNA, indicating the presence of one or more begomoviruses related to SLCMV and the betasatellite, CLCuMB. The begomovirus and betasatellite could be mechanically transmitted to Nicotiana benthamiana. Whitefly transmission of the resident begomovirus could also be demonstrated on mentha. The evidence indicates the association of ToLCKV and CLCuMB, a hitherto new combination of a begomovirus and a betasatellite associated with a leaf deformity disease in mentha in Punjab.Keywords
This publication has 32 references indexed in Scilit:
- Geminivirus strain demarcation and nomenclatureArchiv für die gesamte Virusforschung, 2008
- Recommendations for the classification and nomenclature of the DNA-β satellites of begomovirusesArchiv für die gesamte Virusforschung, 2008
- Identification, detection and transmission of a new vitivirus from MenthaArchiv für die gesamte Virusforschung, 2007
- Differential roles of C4 and βC1 in mediating suppression of post-transcriptional gene silencing: Evidence for transactivation by the C2 of Bhendi yellow vein mosaic virus, a monopartite begomovirusVirus Research, 2007
- A virus between families: nucleotide sequence and evolution of Strawberry latent ringspot virusVirus Research, 2006
- Tomato leaf curl Gujarat virus, a New Begomovirus Species Causing a Severe Leaf Curl Disease of Tomato in Varanasi, IndiaPhytopathology®, 2003
- Detection of Begomoviruses in Clarified Plant Extracts: A Comparison of Standard, Direct-Binding, and Immunocapture Polymerase Chain Reaction TechniquesPhytopathology®, 2003
- Genetic variability of begomoviruses associated with cotton leaf curl disease originating from IndiaArchiv für die gesamte Virusforschung, 2003
- Molecular Characterization of a New Tomato Begomovirus from IndiaPlant Disease, 2003
- Nucleotide sequences from tomato leaf curl viruses from different countries: evidence for three geographically separate branches in evolution of the coat protein of whitefly-transmitted geminivirusesJournal of General Virology, 1995