Conjugative IncFI plasmids carrying CTX-M-15 among Escherichia coliESBL producing isolates at a University hospital in Germany
Open Access
- 17 June 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Infectious Diseases
- Vol. 9 (1), 97
- https://doi.org/10.1186/1471-2334-9-97
Abstract
Multi-drug-resistant, extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, constitute an emerging public-health concern. Little data on the molecular epidemiology of ESBL producing Escherichia coli is available in Germany. Here we describe the prevalence and molecular epidemiology of ESBL producing-Escherichia coli isolates at a German University hospital. We analysed 63 non-duplicate clinical ESBL isolates obtained over an 8-month period using PCR and sequence-based ESBL allele typing, plasmid replicon typing, phylogenetic group typing. Pulsed-field gel electrophoresis (PFGE) based genotyping and plasmid profiling was performed, as well as confirmatory DNA-based hybridization assays. Examination of the 63 Escherichia coli isolates revealed an almost equal distribution among the E. coli phylogenetic groups A, B1, B2 and D. High prevalence (36/63) of the CTX-M-15 gene was observed and an analysis of PFGE-based patterns revealed the presence of this CTX-M allele in multiple clones. Resistance to cefotaxime was a transferable trait and a commonly occurring 145.5 kb conjugative IncFI plasmid was detected in 65% of E. coli carrying the CTX-M-15 allele. The rate of transferable antibiotic resistances for GM, SXT, TET, GM-SXT-TET, SXT-TET and GM-TET was 33%, 61%, 61%, 27%, 44% and 11%, respectively. The remaining strains did not have a common IncFI plasmid but harboured transferable IncFI plasmids with sizes that ranged from 97 to 242.5 kb. Our data demonstrate the presence of IncFI plasmids within the prevailing E. coli population in a hospital setting and suggest that the dissemination of CTX-M-15 allele is associated to lateral transfer of these well-adapted, conjugative IncFI plasmids among various E. coli genotypes.This publication has 22 references indexed in Scilit:
- Dissemination of CTX-M-15 β-Lactamase Genes Carried on Inc FI and FII Plasmids among Clinical Isolates of Escherichia coli in a University Hospital in Istanbul, TurkeyJournal of Clinical Microbiology, 2008
- Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15Emerging Infectious Diseases, 2008
- Molecular Epidemiology of Escherichia coli Producing Extended-Spectrum β-Lactamases Isolated in Rome, ItalyJournal of Clinical Microbiology, 2008
- Rapid Dissemination and Diversity of CTX-M Extended-Spectrum β-Lactamase Genes in Commensal Escherichia coli Isolates from Healthy Children from Low-Resource Settings in Latin AmericaAntimicrobial Agents and Chemotherapy, 2007
- Emergence and Dissemination of Enterobacteriaceae Isolates Producing CTX-M-1-Like Enzymes in Spain Are Associated with IncFII (CTX-M-15) and Broad-Host-Range (CTX-M-1, -3, and -32) PlasmidsAntimicrobial Agents and Chemotherapy, 2007
- Replicon Typing of Plasmids Encoding Resistance to Newer β-LactamsEmerging Infectious Diseases, 2006
- Extended-Spectrum β-Lactamases: a Clinical UpdateClinical Microbiology Reviews, 2005
- Activity of tigecycline tested against a global collection of Enterobacteriaceae, including tetracycline-resistant isolatesDiagnostic Microbiology and Infectious Disease, 2005
- Growing Group of Extended-Spectrum β-Lactamases: the CTX-M EnzymesAntimicrobial Agents and Chemotherapy, 2004
- Comparative Genomic Hybridization: Synchronous Occurrence of Focal Nodular Hyperplasia and Hepatocellular Carcinoma in the Same Liver Is Not Based on Common Chromosomal AberrationsAmerican Journal of Clinical Pathology, 2003