Quantitative expression profile of PSGR in prostate cancer
- 18 October 2005
- journal article
- research article
- Published by Springer Science and Business Media LLC in Prostate Cancer and Prostatic Diseases
- Vol. 9 (1), 56-61
- https://doi.org/10.1038/sj.pcan.4500836
Abstract
PSGR is a novel member of the G-protein-coupled olfactory receptor family. Our initial report showed predominant expression of the PSGR in human prostate gland and significant alterations of PSGR expression in primary prostate cancer (CaP) specimens. The aim of this study was to provide in-depth evaluations of the expression profile of PSGR in prostatic epithelial cells of CaP patients and to evaluate the association of PSGR expression characteristics with clinico-pathologic features. In total, 220 RNA specimens, from laser capture microdissected paired benign and malignant prostatic epithelial cells of 110 CaP patients, were analyzed for PSGR expression by quantitative real-time PCR. The differential expression of PSGR between the prostatic epithelial cells of malignant and benign glands was statistically significant (PPSGR expression between paired benign and tumor cells revealed prostate tumor cell-specific overexpression in 67.2% of tumor specimens (74 of 110), decreased expression in 20.9% of tumor specimens (23 of 110) and no difference of PSGR expression between tumor and normal cells in 11.8% of specimens (13 of 110). In representative cases, PSGR expression patterns were independently confirmed by in situ RNA hybridization. The PSGR overexpression associated with higher percentage of pathologic stage, pT3, and a higher level of preoperative serum PSA. CaP cells of African-American CaP patients exhibited about two-fold increase of PSGR expression in comparison to the Caucasian American CaP patients. Strikingly high-percentage CaP cells overexpress PSGR warrants further studies of PSGR expression alterations to define subsets of CaPs.Keywords
This publication has 25 references indexed in Scilit:
- Cancer Statistics, 2004CA: A Cancer Journal for Clinicians, 2004
- Novel human G-protein-coupled receptorsBiochemical and Biophysical Research Communications, 2003
- Cloning and genetic characterization of an evolutionarily conserved human olfactory receptor that is differentially expressed across speciesGene, 2001
- Identification of a prostate-specific G-protein coupled receptor in prostate cancerOncogene, 2001
- Use of laser capture microdissection, cDNA microarrays, and tissue microarrays in advancing our understanding of prostate cancerThe Journal of Pathology, 2001
- Molecular profiling of tissue samples using laser capture microdissectionExpert Review of Molecular Diagnostics, 2001
- Seven-Transmembrane Proteins as Odorant and Chemosensory ReceptorsScience, 1999
- Laser-capture microdissection: opening the microscopic frontier to molecular analysisTrends in Genetics, 1998
- Update on the diagnosis and treatment of prostate cancerCurrent Opinion in Oncology, 1998
- Survival After Radical ProstatectomyPublished by American Medical Association (AMA) ,1997