Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach
Open Access
- 27 September 2010
- journal article
- Published by Springer Science and Business Media LLC in BMC Genomics
- Vol. 11 (1), 1-16
- https://doi.org/10.1186/1471-2164-11-518
Abstract
Background: Despite rhabdoviral infections being one of the best known fish diseases, the gene expression changes induced at the surface tissues after the natural route of infection (infection-by-immersion) have not been described yet. This work describes the differential infected versus non-infected expression of proteins and immune-related transcripts in fins and organs of zebrafish Danio rerio shortly after infection-by-immersion with viral haemorrhagic septicemia virus (VHSV). Results: Two-dimensional differential gel electrophoresis detected variations on the protein levels of the enzymes of the glycolytic pathway and cytoskeleton components but it detected very few immune-related proteins. Differential expression of immune-related gene transcripts estimated by quantitative polymerase chain reaction arrays and hybridization to oligo microarrays showed that while more transcripts increased in fins than in organs (spleen, head kidney and liver), more transcripts decreased in organs than in fins. Increased differential transcript levels in fins detected by both arrays corresponded to previously described infection-related genes such as complement components (c3b, c8 and c9) or class I histocompatibility antigens (mhc1) and to newly described genes such as secreted immunoglobulin domain (sid4), macrophage stimulating factor (mst1) and a cluster differentiation antigen (cd36). Conclusions: The genes described would contribute to the knowledge of the earliest molecular events occurring in the fish surfaces at the beginning of natural rhabdoviral infections and/or might be new candidates to be tested as adjuvants for fish vaccines.This publication has 60 references indexed in Scilit:
- Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single CellsScience, 2010
- Co‐immunization with an optimized plasmid‐encoded immune stimulatory interleukin, high‐mobility group box 1 protein, results in enhanced interferon‐γ secretion by antigen‐specific CD8 T cellsImmunology, 2009
- The Major Portal of Entry of Koi Herpesvirus in Cyprinus carpio Is the SkinJournal of Virology, 2009
- Mechanism of action of clinically approved adjuvantsCurrent Opinion in Immunology, 2009
- Zebrafish as a model for infectious disease and immune functionFish & Shellfish Immunology, 2008
- Expressed sequence tags (ESTs) from immune tissues of turbot (Scophthalmus maximus) challenged with pathogensBMC Veterinary Research, 2008
- Comparative analysis of the acute response of the trout, O. mykiss, head kidney to in vivo challenge with virulent and attenuated infectious hematopoietic necrosis virus and LPS-induced inflammationBMC Genomics, 2008
- Combined Transcriptome and Proteome Analysis Identifies Pathways and Markers Associated with the Establishment of Rapeseed Microspore-Derived Embryo DevelopmentPlant Physiology, 2007
- Directional responses following recombinant cytokine stimulation of rainbow trout (Oncorhynchus mykiss) RTS-11 macrophage cells as revealed by transcriptome profilingBMC Genomics, 2007
- Evolution of anaphylatoxins, their diversity and novel roles in innate immunity: Insights from the study of fish complementVeterinary Immunology and Immunopathology, 2005