Deletion of a Cation Transporter Promotes Lysis in Streptococcus pneumoniae
- 1 June 2011
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 79 (6), 2314-2323
- https://doi.org/10.1128/iai.00677-10
Abstract
Streptococcus pneumoniae is a significant human pathogen which causes respiratory and serious invasive diseases. Mg 2+ is essential for life, and its concentration varies throughout the human body. Magnesium uptake plays an important role in the virulence of many bacterial pathogens. To study the Mg 2+ uptake of S. pneumoniae strain D39, a mutant was generated in SPD1383, a P-type ATPase with homology to the Salmonella Mg 2+ transporter MgtA, which has also been shown to be a Ca 2+ exporter in strain TIGR4. Under low-Ca 2+ conditions, mutation led to a growth defect in complex medium and the gene was nearly essential for growth under low-Mg 2+ conditions. Addition of Mg 2+ restored the normal growth of the mutant in all cases, but the addition of other divalent cations had no effect. Addition of Ca 2+ , Mn 2+ , and Zn 2+ in the presence of high Mg 2+ concentrations inhibited restoration of growth. The mutant was unable to proliferate in blood, which was also alleviated by the addition of Mg 2+ . The protein was located in the membrane and produced in various S. pneumoniae strains and pathogenic streptococcal species. Surprisingly, mutation of the gene led to an elevated toxicity for endothelial cells. This was caused by an increased amount of pneumolysin in the medium, mediated by elevated lysis of the mutant. Thus, in this study, we uncovered a role for SPD1383 in Mg 2+ uptake and hypothesize that the protein is a Mg 2+/ Ca 2+ antiporter. Furthermore, a disturbance in Mg 2+ homeostasis seems to promote lysis of S. pneumoniae .Keywords
This publication has 58 references indexed in Scilit:
- Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganismsNature Methods, 2009
- TOPCONS: consensus prediction of membrane protein topologyNucleic Acids Research, 2009
- Modulation of Thiol-Disulfide Oxidoreductases for Increased Production of Disulfide-Bond-Containing Proteins in Bacillus subtilisApplied and Environmental Microbiology, 2008
- Calcium efflux is essential for bacterial survival in the eukaryotic hostMolecular Microbiology, 2008
- The cia operon of Streptococcus mutans encodes a unique component required for calcium‐mediated autoregulationMolecular Microbiology, 2008
- A Functional dlt Operon, Encoding Proteins Required for Incorporation of d -Alanine in Teichoic Acids in Gram-Positive Bacteria, Confers Resistance to Cationic Antimicrobial Peptides in Streptococcus pneumoniaeJournal of Bacteriology, 2006
- Identification of a Candidate Streptococcus pneumoniae Core Genome and Regions of Diversity Correlated with Invasive Pneumococcal DiseaseInfection and Immunity, 2006
- Characterization of Phagosome Trafficking and Identification of PhoP-Regulated Genes Important for Survival ofYersinia pestisin MacrophagesInfection and Immunity, 2006
- Pfam: clans, web tools and servicesNucleic Acids Research, 2006
- Multiple sequence alignment with the Clustal series of programsNucleic Acids Research, 2003