PLD2 is enriched on exosomes and its activity is correlated to the release of exosomes
- 14 July 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 572 (1-3), 11-14
- https://doi.org/10.1016/j.febslet.2004.06.082
Abstract
Exosomes are small vesicles secreted by different immune cells and which display anti-tumoral properties. Stimulation of RBL-2H3 cells with ionomycin triggered phospholipase D2 (PLD2) translocation from plasma membrane to intracellular compartments and the release of exosomes. Although exosomes carry the two isoforms of PLD, PLD2 was enriched and specifically sorted on exosomes when overexpressed in cells. PLD activity present on exosomes was clearly increased following PLD2 overexpression. PLD2 activity in cells was correlated to the amount of exosome released, as measured by FACS. Therefore, the present work indicates that exosomes can vehicle signaling enzymes.Keywords
This publication has 23 references indexed in Scilit:
- Mast cell- and dendritic cell-derived exosomes display a specific lipid composition and an unusual membrane organizationBiochemical Journal, 2004
- Disruption of lipid order by short-chain ceramides correlates with inhibition of phospholipase D and downstream signaling by FcϵRIJournal of Cell Science, 2003
- Dual role for phosphoinositides in regulation of yeast and mammalian phospholipase D enzymesThe Journal of cell biology, 2002
- Phospholipase D2 Is Localized to the Rims of the Golgi Apparatus in Mammalian CellsMolecular Biology of the Cell, 2002
- Dynamics of Major Histocompatibility Complex Class II Compartments during B Cell Receptor–mediated Cell ActivationThe Journal of Experimental Medicine, 2002
- Phosphatidic Acid-Mediated Mitogenic Activation of mTOR SignalingScience, 2001
- Actin Directly Interacts with Phospholipase D, Inhibiting Its ActivityJournal of Biological Chemistry, 2001
- Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell derived exosomesNature Medicine, 1998
- Regulation of Phospholipase D2: Selective Inhibition of Mammalian Phospholipase D Isoenzymes by α- and β-SynucleinsBiochemistry, 1998
- ARF and PITP restore GTPγS-stimulated protein secretion from cytosol-depleted HL60 cells by promoting PIP2 synthesisCurrent Biology, 1996