Isolation and characterization of a highly pathogenic strain ofPorcine enteric alphacoronaviruscausing watery diarrhoea and high mortality in newborn piglets
- 13 August 2018
- journal article
- research article
- Published by Hindawi Limited in Transboundary and Emerging Diseases
- Vol. 66 (1), 119-130
- https://doi.org/10.1111/tbed.12992
Abstract
Porcine enteric alphacoronavirus (PEAV) was first discovered in China in February 2017, and the origin and virulence of this novel porcine coronavirus were not fully characterized. Here, we isolated a strain of PEAV, named GDS04 that is identified by immunofluorescence and typical crown‐shaped particles observed with electron microscopy. Genomic analysis reveals that PEAV GDS04 shares a close relationship with SADS‐CoV and SeACoV. Furthermore, newborn piglets orally challenged with PEAV GDS04 developed typical clinical symptoms as watery diarrhoea in neonatal piglets. Viral RNA was detected in faeces and various tissues of the infected piglets. Moreover, macroscopic and microscopic lesions in whole intestinal tract were observed, and viral antigen could be detected in the small intestines by immunohistochemical staining and electron microscopy. Importantly, the mortality rate of inoculated‐newborn piglets was 100% and half of the cohabiting piglets died. Collectively, we demonstrate that PEAV is highly pathogenic in newborn piglets.Keywords
Funding Information
- National Natural Science Foundation of China (31741118)
- Natural Science Foundation of Guangdong Province (2018B030314003)
This publication has 32 references indexed in Scilit:
- Isolation and characterization of a variant porcine epidemic diarrhea virus in ChinaVirology Journal, 2012
- Discovery of Seven Novel Mammalian and Avian Coronaviruses in the Genus Deltacoronavirus Supports Bat Coronaviruses as the Gene Source of Alphacoronavirus and Betacoronavirus and Avian Coronaviruses as the Gene Source of Gammacoronavirus and DeltacoronavirusJournal of Virology, 2012
- Genetic Diversity of Avian Infectious Bronchitis Virus Isolated from Domestic Chicken Flocks and Coronaviruses from Feral Pigeons in Brazil Between 2003 and 2009Avian Diseases, 2010
- Coronavirus Genomics and Bioinformatics AnalysisViruses, 2010
- Proteomic analysis of purified coronavirus infectious bronchitis virus particlesProteome Science, 2010
- The GPRLQPY motif located at the carboxy-terminal of the spike protein induces antibodies that neutralize Porcine epidemic diarrhea virusVirus Research, 2008
- Evolutionary Relationships between Bat Coronaviruses and Their HostsEmerging Infectious Diseases, 2007
- Evidence for a Porcine Respiratory Coronavirus, Antigenically Similar to Transmissible Gastroenteritis Virus, in the United StatesJournal of Veterinary Diagnostic Investigation, 1990
- Isolation of rhinoviruses and coronaviruses from 38 colds in adultsJournal of Medical Virology, 1980
- A new coronavirus-like particle associated with diarrhea in swineArchiv für die gesamte Virusforschung, 1978