Platycodin D Improves the Immunogenicity of Newcastle Disease Virus‐Based Recombinant Avian Influenza Vaccine in Mice
- 15 March 2010
- journal article
- research article
- Published by Wiley in Chemistry & Biodiversity
- Vol. 7 (3), 677-689
- https://doi.org/10.1002/cbdv.200900183
Abstract
The development of an effective influenza vaccine is urgently important for controlling outbreaks of the highly pathogenic avian influenza virus (HPAIV) and reducing the impact of pandemics. The use of an adjuvant in such a vaccine can significantly contribute to improve the immunogenicity. To explore a novel and safe adjuvant for improving the potency of influenza vaccines, platycodin D (1), a saponin from the root of Platycodon grandiflorum, was evaluated for the adjuvant potentials on the specific cellular and humoral immune responses to Newcastle disease virus-based recombinant avian influenza vaccine (rL-H5) in mice. Compound 1 significantly promoted the concanavalin A (Con A)-, the lipopolysaccharide (LPS)-, and the antigen-induced splenocyte proliferation and enhanced the serum antigen-specific IgG, IgG1, IgG2a, and IgG2b antibody titers (PPPγ and IL-10) and transcription factors (T-bet and GATA-3) in splenocytes were also markedly up-regulated by 1, compared with the control group immunized with rL-H5 alone (PPP<0.05), which may have important implications for the vaccination against the avian influenza virus. We concluded that 1 could improve the immunogenicity of the rL-H5 vaccine by enhancing both humoral and cellular immune responses in mice, and that 1 is a promising adjuvant for influenza vaccines.This publication has 59 references indexed in Scilit:
- Formation and function of the lytic NK-cell immunological synapseNature Reviews Immunology, 2008
- Immunization of Primates with a Newcastle Disease Virus-Vectored Vaccine via the Respiratory Tract Induces a High Titer of Serum Neutralizing Antibodies against Highly Pathogenic Avian Influenza VirusJournal of Virology, 2007
- Humoral and cell-mediated immune responses of humans to inactivated influenza vaccine with or without QS21 adjuvantVaccine, 2007
- Protective avian influenza in ovo vaccination with non-replicating human adenovirus vectorVaccine, 2007
- Avian Influenza Virus (H5N1): a Threat to Human HealthClinical Microbiology Reviews, 2007
- Newcastle disease virus expressing H5 hemagglutinin gene protects chickens against Newcastle disease and avian influenzaProceedings of the National Academy of Sciences of the United States of America, 2006
- Evolution of H5N1 Avian Influenza Viruses in AsiaEmerging Infectious Diseases, 2005
- Immunogenicity of a monovalent, aluminum-adjuvanted influenza whole virus vaccine for pandemic useVirus Research, 2004
- PrefacePhilosophical Transactions B, 2001
- An ELISA for detection of antibodies against influenza A nucleoprotein in humans and various animal speciesArchiv für die gesamte Virusforschung, 1990