Phosphorylation of Microtubule‐Associated Proteins
- 1 March 1976
- journal article
- Published by Wiley in JBIC Journal of Biological Inorganic Chemistry
- Vol. 62 (3), 539-549
- https://doi.org/10.1111/j.1432-1033.1976.tb10188.x
Abstract
1. Tubulin is not an adenosine-3':5'-monophosphate-dependent (cyclic-AMP-dependent) protein kinase. Both entities have been clearly separated by sucrose gradient ultracentrifugation. With a tubulin preparation obtained by the polymerization-depolymerization technique protein kinase had a sedimentation coefficient of 8.7 S whereas tubulin sedimented with 6.4 S. After preincubation with both cyclic AMP and histone the kinase dissociated into its catalytic subunit with a sedimentation coefficient of 3.4 S. 2. Tubulin prepared by the polymerization-depolymerization technique was neither phosphorylated in vivo nor in vitro. On the contrary if this preparation was further purified by the Weisenberg's procedure (DEAE-Sephadex batch absorption) before incubation with [gamma-32 P]ATP, phosphorylation occurred. Thus, phosphorylation depended on the method used to purify tubulin i.e. was likely to an an artefact.Keywords
This publication has 60 references indexed in Scilit:
- Resolution of cyclic AMP stimulated protein kinase from polymerization-purified brain microtubulesBiochemical and Biophysical Research Communications, 1975
- Evidence for firm linkages between microtubules and membrane-bounded vesicles.The Journal of cell biology, 1975
- Direct biochemical measurements of microtubule assembly and disassembly in Chinese hamster ovary cells. The effect of intercellular contact, cold, D2O, and N6,O2'-dibutyryl cyclic adenosine monophosphate.The Journal of cell biology, 1975
- The presence of phospholipids and diglyceride kinase activity in microtubules from different tissuesBiochemical and Biophysical Research Communications, 1974
- Neurotubulin polymerization and phosphorylation reactions catalyzed by ‘associated’ protein kinaseFEBS Letters, 1974
- Phosphorylation of Vinblastine‐Isolated Microtubules from Chick‐Embryonic MusclesJBIC Journal of Biological Inorganic Chemistry, 1974
- A dynein‐like protein associated with neurotubulesFEBS Letters, 1974
- Phosphorylation and aggregation of neurotubulin and ‘associated’ protein-kinaseMolecular and Cellular Endocrinology, 1974
- Interactions of tubulin with vinblastine and guanosine triphosphateJournal of Molecular Biology, 1972
- Amino acid and energy requirements of protein synthesis in rat pancreatic tissue in vitroBiochimica et Biophysica Acta, 1962