Sensitivity of the Quidel Sofia Fluorescent Immunoassay Compared With 2 Nucleic Acid Assays and Viral Culture to Detect Pandemic Influenza A(H1N1)pdm09
Open Access
- 1 August 2015
- journal article
- research article
- Published by Oxford University Press (OUP) in Laboratory Medicine
- Vol. 46 (3), 230-234
- https://doi.org/10.1309/lmrxbn8p59itdhqz
Abstract
To confirm a diagnosis of influenza at the point of care, healthcare professionals may rely on rapid influenza diagnostic tests (RIDTs). RIDTs have low to moderate sensitivity compared with viral culture or real-time reverse-transcription polymerase chain reaction (rRT-PCR). With the resurgence of the influenza A (Flu A; subtype H1N1) pandemic 2009 (pdm09) strain in the years 2013 and 2014, we evaluated the accuracy of the United State Food and Drug Administration (FDA)-approved Sofia Influenza A+B Fluorescent Immunoassay to detect epidemic Flu A(H1N1)pdm09 in specimens from the upper-respiratory tract. During a 3-month period, we collected 40 specimens that tested positive via PCR and/or culture for Flu A of the H1N1 pdm09 subtype. Of the 40 specimens, 27 tested positive (67.5%) via Sofia assay for Flu A. Of the 13 specimens with a negative result via Sofia testing, 4 had coinfection, as detected by the GenMark Diagnostics eSensor Respiratory Viral Panel. This sensitivity of the RIDT Sofia assay to detect Flu A(H1N1) pdm09 was comparable to previously reported sensitivities ranging from 10% to 75% for older RIDTs.Keywords
This publication has 15 references indexed in Scilit:
- Rapid influenza diagnostic test use and antiviral prescriptions in outpatient settings pre- and post-2009 H1N1 pandemicJournal of Clinical Virology, 2014
- Efficacy of oseltamivir treatment started within 5 days of symptom onset to reduce influenza illness duration and virus shedding in an urban setting in Bangladesh: a randomised placebo-controlled trialThe Lancet Infectious Diseases, 2013
- Comparison of conventional lateral-flow assays and a new fluorescent immunoassay to detect influenza virusesJournal of Virological Methods, 2013
- Detection of Influenza A and B Viruses With the Sofia AnalyzerAmerican Journal of Clinical Pathology, 2013
- Quantitative analysis of four rapid antigen assays for detection of pandemic H1N1 2009 compared with seasonal H1N1 and H3N2 influenza A viruses on nasopharyngeal aspirates from patients with influenzaJournal of Virological Methods, 2012
- Evaluation of Sofia fluorescent immunoassay analyzer for influenza A/B virusJournal of Clinical Virology, 2012
- Evaluation of Indirect Fluorescent Antibody Assays Compared to Rapid Influenza Diagnostic Tests for the Detection of Pandemic Influenza A (H1N1) pdm09PLOS ONE, 2012
- Very low pandemic influenza A (H1N1) 2009 mortality associated with early neuraminidase inhibitor treatment in Japan: Analysis of 1000 hospitalized childrenJournal of Infection, 2011
- Diagnostic Accuracy of a Rapid Influenza Test for Pandemic Influenza A H1N1PLOS ONE, 2010
- Comparison of a Rapid Antigen Test with Nucleic Acid Testing during Cocirculation of Pandemic Influenza A/H1N1 2009 and Seasonal Influenza A/H3N2Journal of Clinical Microbiology, 2010