Chromosomal localization of human O6-methylguanine-DNA methyltransferase (MGMT) gene by in situ hybridization

Abstract

Recombinant lambda phage DNA containing segments of human O⁶-methylguanine-DNA transferase gene was employed to localize this gene among the human chromosomes using noii-radioactive in situ hybridization technique. This gene was found to be present at the telomeric end, 26q, of the long arm of the chromosome 10.