The Prp19p-Associated Complex in Spliceosome Activation
- 10 October 2003
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 302 (5643), 279-282
- https://doi.org/10.1126/science.1086602
Abstract
During spliceosome activation, a large structural rearrangement occurs that involves the release of two small nuclear RNAs, U1 and U4, and the addition of a protein complex associated with Prp19p. We show here that the Prp19p-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is dissociated. Ultraviolet crosslinking analysis revealed the existence of two modes of base pairing between U6 and the 5′ splice site, as well as a switch of such base pairing from one to the other that required the Prp19p-associated complex during spliceosome activation. Moreover, a Prp19p-dependent structural change in U6 small nuclear ribonucleoprotein particles was detected that involves destabilization of Sm-like (Lsm) proteins to bring about interactions between the Lsm binding site of U6 and the intron sequence near the 5′ splice site, indicating dynamic association of Lsm with U6 and a direct role of Lsm proteins in activation of the spliceosome.Keywords
This publication has 22 references indexed in Scilit:
- The Clf1p Splicing Factor Promotes Spliceosome Assembly through N-terminal Tetratricopeptide Repeat ContactsPublished by Elsevier BV ,2003
- Small Nuclear Ribonucleoprotein Remodeling During Catalytic Activation of the SpliceosomeScience, 2002
- Allosteric Cascade of Spliceosome ActivationAnnual Review of Genetics, 2002
- Comprehensive proteomic analysis of the human spliceosomeNature, 2002
- Proteomics Analysis Reveals Stable Multiprotein Complexes in Both Fission and Budding Yeasts Containing Myb-Related Cdc5p/Cef1p, Novel Pre-mRNA Splicing Factors, and snRNAsMolecular and Cellular Biology, 2002
- Identification and Characterization of Two Novel Components of The Prp19p-associated Complex, Ntc30p and Ntc20pPublished by Elsevier BV ,2001
- An RNA Switch at the 5′ Splice Site Requires ATP and the DEAD Box Protein Prp28pMolecular Cell, 1999
- The U5 and U6 Small Nuclear RNAs as Active Site Components of the SpliceosomeScience, 1993
- Mutations in U6 snRNA that Alter Splice Site Specificity: Implications for the Active SiteScience, 1993
- Association of U6 snRNA with the 5'-splice site region of pre-mRNA in the spliceosome.Genes & Development, 1992