Pulsed-Field Gel Electrophoresis of Staphylococcus aureus Isolates from Atopic Patients Revealing Presence of Similar Strains in Isolates from Children and Their Parents
- 1 February 2008
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 46 (2), 456-461
- https://doi.org/10.1128/jcm.01734-07
Abstract
Skin colonization with Staphylococcus aureus is often associated with atopic dermatitis, and staphylococcal enterotoxins have been implicated in the etiology of atopic disease. In this study, the colonization of patients with atopic dermatitis and their parents was investigated in order to evaluate the possibility of intrafamiliar transmission. S. aureus strains were isolated from 30 of 45 patients (66%). In 19 of 29 families (65%), at least one parent carried S. aureus , and the overall colonization rate of the parents was 48%. All strains were typed by pulsed-field gel electrophoresis (PFGE), and the presence of enterotoxin genes in the strains was assayed by multiplex PCR. A high percentage (84%) of the isolates present on the children and on at least one of their parents displayed identical PFGE and enterotoxin patterns as well as identical antibiotic resistance profiles, indicating intrafamiliar transmission. Forty-five percent of the strains did not carry any enterotoxin gene. The most frequently found enterotoxin genes were seg and sei , which were present in 36% of the strains, and seb , which was found in 24% of the strains. The other toxin genes occurred only in low frequencies. Most strains were resistant to penicillin (82%), and 15% showed resistance to more than one antibiotic. Intermediately-vancomycin-resistant S. aureus or methicillin-resistant S. aureus strains were not detected. In conclusion, this study indicates that the colonization rate of parents of atopic children is rather high and may increase the risk of recolonization of the child.Keywords
This publication has 38 references indexed in Scilit:
- Bacterial superantigen facilitates epithelial presentation of allergen to T helper 2 cellsProceedings of the National Academy of Sciences of the United States of America, 2007
- Biotyping of Enterotoxigenic Staphylococcus aureus by Enterotoxin Gene Cluster ( egc ) Polymorphism and spa Typing AnalysesApplied and Environmental Microbiology, 2006
- Skin colonization byStaphylococcus aureusin patients with eczema and atopic dermatitis and relevant combined topical therapy: a double-blind multicentre randomized controlled trialBritish Journal of Dermatology, 2006
- Prevalence of Toxic Shock Syndrome Toxin 1-Producing Staphylococcus aureus and the Presence of Antibodies to This Superantigen in Menstruating WomenJournal of Clinical Microbiology, 2005
- Staphylococcal toxins in patients with psoriasis, atopic dermatitis, and erythroderma, and in healthy control subjectsJournal of the American Academy of Dermatology, 2005
- Persistent skin colonization with Staphylococcus aureus in atopic dermatitis: relationship to clinical and immunological parametersClinical & Experimental Allergy, 2005
- Staphylococcal enterotoxin‐specific IgE antibodies in atopic dermatitisPediatrics International, 2004
- Presence of staphylococcal exfoliative toxin A in sera of patients with atopic dermatitisClinical & Experimental Allergy, 2004
- Preferential Binding of Staphylococcus aureus to Skin Sites of Th2-Mediated Inflammation in a Murine ModelJournal of Investigative Dermatology, 2001
- Membrane mutations and production of enterotoxin B and alpha hemolysin in Staphylococcus aureusCanadian Journal of Microbiology, 1975