Ribotyping and Randomly Amplified Polymorphic DNA Analysis of Vibrio vulnificus Biotypes
- 31 July 1993
- journal article
- Published by Elsevier BV in Systematic and Applied Microbiology
- Vol. 16 (2), 303-309
- https://doi.org/10.1016/s0723-2020(11)80484-4
Abstract
Ribotyping and the randomly amplified polymorphic DNA (RAPD) technique were evaluated as tools for epidemiological studies of Vibrio vulnificus. Ribopatterns of DNA digested with four restriction enzymes and hybridized with two oligonucleotides complementary to highly conserved sequences in the 16S and 23S rRNA genes showed different levels of discrimination among the strains tested. In addition, the presence of eight to ten rRNA operons was revealed in Vibrio strains. Strains of V. vulnificus biotypes 1 and 2 could be differentiated by random amplification of DNA with M13, T3 or T7 universal primers. Compared with ribotyping, RAPD appeared to be a faster method for diagnostic identification of V. vulnificus biotypes since a single colony could be used as a source of DNA template and the final result was obtained within 24 h after sampling. Further, RAPD analysis allowed the identification of individual strains.Keywords
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