Foxl1‐Cre BAC transgenic mice: a new tool for gene ablation in the gastrointestinal mesenchyme
- 27 July 2007
- Vol. 45 (8), 518-522
- https://doi.org/10.1002/dvg.20315
Abstract
The use of Cre‐loxP technology for the purpose of cell type‐specific gene ablation has revolutionized developmental biology and biomedicine. Several transgenic mouse lines have been developed for the analysis of gene function in the gastrointestinal tract, but in all of these the expression of Cre is limited to the epithelial cell layer. No Cre‐ expressing transgenic mouse lines (“Cre lines”) exist for the deletion of loxP‐flanked genes specifically in gut mesoderm. To address this deficiency, we have derived a bacterial artificial chromosome based transgenic mouse line in which the Cre gene is controlled by the Foxl1 promoter and enhancer elements. X‐Gal staining of Foxl1‐Cre; Rosa26R bi‐transgenic lines confirm that Foxl1‐Cre results in recombination specifically in the gastrointestinal mesenchyme. The Foxl1‐Cre line will facilitate the dissection of mesenchymal to epithelial signaling that is known to play a major role in the patterning and function of the gastrointestinal tract. genesis 45:518–522, 2007. Published 2007 Wiley‐Liss, Inc.Keywords
This publication has 26 references indexed in Scilit:
- Foxl1-deficient mice exhibit aberrant epithelial cell positioning resulting from dysregulated EphB/EphrinB expression in the small intestineAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2006
- Foxf1andFoxf2control murine gut development by limiting mesenchymal Wnt signaling and promoting extracellular matrix productionDevelopment, 2006
- Foxa2 is required for the differentiation of pancreatic α-cellsDevelopmental Biology, 2004
- Reduction of SNAP25 in acid secretion defect of Foxl1−/− gastric parietal cellsBiochemical and Biophysical Research Communications, 2004
- A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout MutationsGenome Research, 2003
- cis Elements of the Villin Gene Control Expression in Restricted Domains of the Vertical (Crypt) and Horizontal (Duodenum, Cecum) Axes of the IntestineJournal of Biological Chemistry, 2002
- Mesenchymal Transcription Factor Fkh6 Is Essential for the Development and Differentiation of Parietal CellsBiochemical and Biophysical Research Communications, 2001
- Chromosomal Duplication Involving the Forkhead Transcription Factor GeneFOXC1Causes Iris Hypoplasia and GlaucomaAmerican Journal of Human Genetics, 2000
- Mesenchyme-Dependent Differentiation of Epithelial Progenitor Cells in the GutJournal of Pediatric Gastroenterology and Nutrition, 1987
- Fetal gut mesenchyme induces differentiation of cultured intestinal endodermal and crypt cellsDevelopmental Biology, 1986