Chromatin Boundaries in Budding Yeast: The Nuclear Pore Connection
- 31 May 2002
- journal article
- research article
- Published by Elsevier BV in Cell
- Vol. 109 (5), 551-562
- https://doi.org/10.1016/s0092-8674(02)00756-0
Abstract
Chromatin boundary activities (BAs) were identified in Saccharomyces cerevisiae by genetic screening. Such BAs bound to sites flanking a reporter gene establish a nonsilenced domain within the silent mating-type locus HML. Interestingly, various proteins involved in nuclear-cytoplasmic traffic, such as exportins Cse1p, Mex67p, and Los1p, exhibit a robust BA. Genetic studies, immunolocalization, live imaging, and chromatin immunoprecipitation experiments show that these transport proteins block spreading of heterochromatin by physical tethering of the HML locus to the Nup2p receptor of the nuclear pore complex. Genetic deletion of NUP2 abolishes the BA of all transport proteins, while direct targeting of Nup2p to the bracketing DNA elements restores activity. The data demonstrate that physical tethering of genomic loci to the NPC can dramatically alter their epigenetic activity.Keywords
This publication has 49 references indexed in Scilit:
- Silenced Chromatin Is Permissive to Activator Binding and PIC RecruitmentCell, 2001
- Transport Between the Cell Nucleus and the CytoplasmAnnual Review of Cell and Developmental Biology, 1999
- The Yeast Nucleoporin Nup2p Is Involved in Nuclear Export of Importin α/Srp1pPublished by Elsevier BV ,1999
- NUCLEOCYTOPLASMIC TRANSPORT: The Soluble PhaseAnnual Review of Biochemistry, 1998
- Silencing and Heritable Domains of Gene ExpressionAnnual Review of Cell and Developmental Biology, 1995
- Visualization of chromosomal domains with boundary element-associated factor BEAF-32Cell, 1995
- Overcoming telomeric silencing: a trans-activator competes to establish gene expression in a cell cycle-dependent way.Genes & Development, 1994
- A position-effect assay for boundaries of higher order chromosomal domainsCell, 1991
- The 87A7 chromomere: Identification of novel chromatin structures flanking the heat shock locus that may define the boundaries of higher order domainsJournal of Molecular Biology, 1985
- A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistanceMolecular Genetics and Genomics, 1984