Effect of 2-wk intensified training and inactivity on muscle Na+-K+pump expression, phospholemman (FXYD1) phosphorylation, and performance in soccer players
- 1 April 2010
- journal article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 108 (4), 898-905
- https://doi.org/10.1152/japplphysiol.01015.2009
Abstract
The present study examined muscle adaptations and alterations in performance of highly trained soccer players with intensified training or training cessation. Eighteen elite soccer players were, for a 2-wk period, assigned to either a group that performed high-intensity training with a reduction in the amount of training (HI, n = 7), or an inactivity group without training (IN, n = 11). HI improved ( P < 0.05) performance of the 4th, 6th, and 10th sprint in a repeated 20-m sprint test, and IN reduced ( P < 0.05) performance in the 5th to the 10th sprints after the 2-wk intervention period. In addition, the Yo-Yo intermittent recovery level 2 test performance of IN was lowered from 845 ± 48 to 654 ± 30 m. In HI, the protein expression of the Na+-K+pump α2-isoform was 15% higher ( P < 0.05) after the intervention period, whereas no changes were observed in α1- and β1-isoform expression. In IN, Na+-K+pump expression was not changed. In HI, the FXYD1ser68-to-FXYD1 ratio was 27% higher ( P < 0.01) after the intervention period, and, in IN, the AB_FXYD1ser68 signal was 18% lower ( P < 0.05) after inactivity. The change in FXYD1ser68-to-FXYD1 ratio was correlated ( r2= 0.35; P < 0.05) with change in performance in repeated sprint test. The present data suggest that short-term intensified training, even for trained soccer players, can increase muscle Na+-K+pump α2-isoform expression, and that cessation of training for 2 wk does not affect the expression of Na+-K+pump isoforms. Resting phosphorylation status of the Na+-K+pump is changed by training and inactivity and may play a role in performance during repeated, intense exercise.Keywords
This publication has 28 references indexed in Scilit:
- Reduced volume and increased training intensity elevate muscle Na+-K+pump α2-subunit expression as well as short- and long-term work capacity in humansJournal of Applied Physiology, 2009
- Altered expression and insulin-induced trafficking of Na+-K+-ATPase in rat skeletal muscle: effects of high-fat diet and exerciseAmerican Journal of Physiology-Endocrinology and Metabolism, 2009
- Do multiple ionic interactions contribute to skeletal muscle fatigue?Journal Of Physiology-London, 2008
- Effect of dexamethasone on skeletal muscle Na+,K+pump subunit specific expression and K+homeostasis during exercise in humansJournal Of Physiology-London, 2008
- The Yo-Yo Intermittent Recovery TestSports Medicine, 2008
- Muscle Na+-K+-ATPase activity and isoform adaptations to intense interval exercise and training in well-trained athletesJournal of Applied Physiology, 2007
- Serine 68 Phospholemman Phosphorylation during Forskolin-Induced Swine Carotid Artery RelaxationJournal of Vascular Research, 2005
- Serine 68 phosphorylation of phospholemman: acute isoform-specific activation of cardiac Na/K ATPaseCardiovascular Research, 2005
- Effect of high-intensity intermittent training on lactate and H+ release from human skeletal muscleAmerican Journal of Physiology-Endocrinology and Metabolism, 2004
- Effect of training intensity on muscle lactate transporters and lactate threshold of cross‐country skiersActa Physiologica Scandinavica, 2001