Quantitative PCR on 5 genes reliably identifies CTCL patients with 5% to 99% circulating tumor cells with 90% accuracy
Open Access
- 15 April 2006
- journal article
- Published by American Society of Hematology in Blood
- Vol. 107 (8), 3189-3196
- https://doi.org/10.1182/blood-2005-07-2813
Abstract
We previously identified a small number of genes using cDNA arrays that accurately diagnosed patients with Sézary Syndrome (SS), the erythrodermic and leukemic form of cutaneous T-cell lymphoma (CTCL). We now report the development of a quantitative real-time polymerase chain reaction (qRT-PCR) assay that uses expression values for just 5 of those genes: STAT4, GATA-3, PLS3, CD1D, and TRAIL. qRT-PCR data from peripheral blood mononuclear cells (PBMCs) accurately classified 88% of 17 patients with high blood tumor burden and 100% of 12 healthy controls in the training set using Fisher linear discriminant analysis (FLDA). The same 5 genes were then assayed on 56 new samples from 49 SS patients with blood tumor burdens of 5% to 99% and 69 samples from 65 new healthy controls. The average accuracy over 1000 resamplings was 90% using FLDA and 88% using support vector machine (SVM). We also tested the classifier on 14 samples from patients with CTCL with no detectable peripheral involvement and 3 patients with atopic dermatitis with severe erythroderma. The accuracy was 100% in identifying these samples as non-SS patients. These results are the first to demonstrate that gene expression profiling by quantitative PCR on a selected number of critical genes can be employed to molecularly diagnosis SS.Keywords
This publication has 70 references indexed in Scilit:
- TRAIL-induced apoptosis in gliomas is enhanced by Akt-inhibition and is independent of JNK activationApoptosis, 2005
- Prediction of Survival in Diffuse Large-B-Cell Lymphoma Based on the Expression of Six GenesNew England Journal of Medicine, 2004
- Mycosis fungoides shows concurrent deregulation of multiple genes involved in the TNF signaling pathway: an expression profile studyBlood, 2003
- Gene-expression profiles predict survival of patients with lung adenocarcinomaNature Medicine, 2002
- Gene Expression Profiling of Lymphoid MalignanciesAnnual Review of Medicine, 2002
- Differential Expression of the L-Plastin Gene in Human Colorectal Cancer Progression and MetastasisBiochemical and Biophysical Research Communications, 2001
- Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implicationsProceedings of the National Academy of Sciences, 2001
- In vitro susceptibility to TRAIL-induced apoptosis of acute leukemia cells in the context of TRAIL receptor gene expression and constitutive NF-κB activityLeukemia, 2001
- Keratinocytes express dipeptidyl-peptidase IV (CD26) in benign and malignant skin diseasesBritish Journal of Dermatology, 1996
- THE STATISTICAL UTILIZATION OF MULTIPLE MEASUREMENTSAnnals of Eugenics, 1938