Activation by Gene Amplification of pitB , Encoding a Third Phosphate Transporter of Escherichia coli K-12
- 1 August 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (15), 4659-4663
- https://doi.org/10.1128/jb.183.15.4659-4663.2001
Abstract
Two systems for the uptake of inorganic phosphate (P i ) in Escherichia coli , PitA and Pst, have been described. A revertant of a pitA pstS double mutant that could grow on P i was isolated. We demonstrate that the expression of a new P i transporter, PitB, is activated in this strain by a gene amplification event.Keywords
This publication has 28 references indexed in Scilit:
- The Complete Genome Sequence of Escherichia coli K-12Science, 1997
- ABC Transporters: From Microorganisms to ManAnnual Review of Cell Biology, 1992
- Expression ofEscherichia coliPhoE protein in avirulentSalmonella typhimurium aroAandgalEstrainsFEMS Microbiology Letters, 1988
- Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vectorGene, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Hyperproduction of Phosphate‐Binding Protein, phoS, and pre‐phoS Proteins in Escherichia coli Carrying a Cloned phoS GeneEuropean Journal of Biochemistry, 1983
- Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and MuJournal of Molecular Biology, 1976
- Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bandsFEBS Letters, 1975
- Arsenate resistant mutants of and phosphate transportBiochemical and Biophysical Research Communications, 1970
- Influence of inorganic phosphate in the formation of phosphatases by Escherichia coliBiochimica et Biophysica Acta, 1959