Tetramethoxy Hydroxyflavone p7F Downregulates Inflammatory Mediators via the Inhibition of Nuclear Factor κB
- 1 December 2004
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 1030 (1), 555-568
- https://doi.org/10.1196/annals.1329.065
Abstract
Artemisia has been traditionally used in Korean herbal medicine to clear damp heat and to treat uteritis and jaundice. Flavonoids isolated from Artemisia are also known to possess anti-inflammatory activities. In this study, 5,6,3',5'-tetramethoxy 7,4'-hydroxyflavone (p7F) was isolated from Artemisia absinthium. We examined in vitro and in vivo regulatory functions of p7F on the production of nitric oxide (NO), prostaglandin E(2) (PGE(2)), and tumor necrosis factor-alpha (TNF-alpha) as well as the expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and collagen-induced arthritis. p7F inhibited the expression or production of proinflammatory mediators such as COX-2/PGE(2) and iNOS/NO in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. p7F also suppressed the serum level of TNF-alpha in mice treated with collagen and inhibited nuclear factor-kappaB (NF-kappaB) activation as well as NF-kappaB promoter activity in RAW 264.7 cells stimulated with LPS. This compound directly inhibited the intracellular accumulation of reactive oxygen species in hydrogen peroxide-stimulated RAW 264.7 cells. p7F has antioxidant activity and inhibits NF-kappaB activation. Taken together, these results suggest that p7F can be clinically applied to the treatment of inflammatory diseases.Keywords
This publication has 27 references indexed in Scilit:
- INHIBITION OF CARCINOGENESIS BY DIETARY POLYPHENOLIC COMPOUNDSAnnual Review of Nutrition, 2001
- Interleukin-1β, Interleukin-5, Interleukin-6, Interleukin-8, and Tumor Necrosis Factor-α in Chronic Sinusitis: Response to Systemic CorticosteroidsAmerican Journal of Rhinology, 2000
- Effect of Bioflavonoids Extracted from the Bark of Pinus maritima on Proinflammatory Cytokine Interleukin-1 Production in Lipopolysaccharide-Stimulated RAW 264.7Toxicology and Applied Pharmacology, 2000
- THE NF-κB AND IκB PROTEINS: New Discoveries and InsightsAnnual Review of Immunology, 1996
- Synergistic Effect of Interleukin-1β and Tumor Necrosis Factor α on PGE2Production by Articular Chondrocytes Does Not Involve PLA2StimulationExperimental Cell Research, 1996
- Composition of Polyphenols in Fresh Tea Leaves and Associations of Their Oxygen-Radical-Absorbing Capacity with Antiproliferative Actions in Fibroblast CellsJournal of Agricultural and Food Chemistry, 1996
- Tea and CancerJNCI Journal of the National Cancer Institute, 1993
- Antioxidative effect of polyphenol extract prepared from various Chinese teasPreventive Medicine, 1992
- DNA Deaminating Ability and Genotoxicity of Nitric Oxide and its ProgenitorsScience, 1991
- Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediateBiochemistry, 1988