Effects of confinement on the mechanical properties of self‐assembled articular cartilage constructs in the direction orthogonal to the confinement surface
Open Access
- 29 August 2007
- journal article
- research article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 26 (2), 238-246
- https://doi.org/10.1002/jor.20480
Abstract
This study examined the effects of radial confinement and passive axial compression‐induced vertical confinement, on the biomechanical, biochemical, and histological properties of self‐assembled chondrocyte constructs. The self‐assembled constructs, engineered without the use of an exogenous scaffold, exhibited significant increases in stiffness in the direction orthogonal to that of the confinement surface. With radial confinement, the significantly increased aggregate modulus was accompanied by increased collagen organization in the direction perpendicular to the articular surface, with no change in collagen or glycosaminoglycan (GAG) content. Additionally, radial confinement was most beneficial when applied before 2 weeks. With passive axial compression, the significantly increased Young's modulus and ultimate tensile strength were accompanied by a significant increase in collagen production. This study is the first to demonstrate the beneficial effects of confinement on tissue engineered constructs in the direction orthogonal to that of the confinement surface. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:238–246, 2008Keywords
This publication has 35 references indexed in Scilit:
- Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded culturesJournal of Biomechanics, 2006
- Increased accumulation of superficial zone protein (SZP) in articular cartilage in response to bone morphogenetic protein‐7 and growth factorsJournal of Orthopaedic Research, 2006
- Long-Term Intermittent Compressive Stimulation Improves the Composition and Mechanical Properties of Tissue-Engineered CartilageTissue Engineering, 2004
- Effect of Low Oxygen Tension on Tissue-Engineered Cartilage Construct Development in the Concentric Cylinder BioreactorTissue Engineering, 2004
- Long‐term intermittent shear deformation improves the quality of cartilaginous tissue formed in vitroJournal of Orthopaedic Research, 2003
- Combining Chondrocytes and Smooth Muscle Cells to Engineer Hybrid Soft Tissue ConstructsTissue Engineering, 2000
- Chondrocyte Migration to Fibronectin, Type I Collagen, and Type II Collagen.Cell Structure and Function, 1997
- Time‐dependent changes in the response of cartilage to static compression suggest interstitial pH is not the only signaling mechanismJournal of Orthopaedic Research, 1995
- Biphasic Creep and Stress Relaxation of Articular Cartilage in Compression: Theory and ExperimentsJournal of Biomechanical Engineering, 1980
- The relation between load and penetration in the axisymmetric boussinesq problem for a punch of arbitrary profileInternational Journal of Engineering Science, 1965