Characterization of Helicobacter pylori Lytic Transglycosylases Slt and MltD
- 15 January 2007
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 189 (2), 422-429
- https://doi.org/10.1128/jb.01270-06
Abstract
Peptidoglycan (PG) is a cell wall heteropolymer that is essential for cell integrity. PG hydrolases participate in correct assembly of the PG layer and have been shown to be required for cell division, cell daughter separation, and maintenance of bacterial morphology. In silico analysis of the Helicobacter pylori genome resulted in identification of three potential hydrolases, Slt, MltD, and AmiA. This study was aimed at determining the roles of the putative lytic transglycosylases, Slt and MltD, in H. pylori morphology, growth, and PG metabolism. Strain 26695 single mutants were constructed using a nonpolar kanamycin cassette. The slt and mltD mutants formed normal bacillary and coccoid bacteria in the exponential and stationary phases, respectively. The slt and mltD mutants had growth rates comparable to the growth rate of the parental strain. However, the mltD mutant exhibited enhanced survival in the stationary phase compared to the wild type or the slt mutant. PG was purified from exponentially growing bacteria and from bacteria in the stationary phase, and its muropeptide composition was analyzed by high-pressure liquid chromatography. This analysis revealed changes in the muropeptide composition indicating that MltD and Slt have lytic transglycosylase activities. Glycan strand analysis suggested that Slt and MltD have exo and endo types of lytic transglycosylase activity, indicating that Slt is involved mainly in PG turnover and MltD is involved mainly in rearrangement of the PG layer. In this study, we determined the distinct roles of the lytic transglycosylases Slt and MltD in PG metabolism.Keywords
This publication has 23 references indexed in Scilit:
- Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune EscapePLoS Pathogens, 2006
- Role ofmurFin Cell Wall Biosynthesis: Isolation and Characterization of amurFConditional Mutant ofStaphylococcus aureusJournal of Bacteriology, 2006
- Responsiveness to acidity via metal ion regulators mediates virulence in the gastric pathogen Helicobacter pyloriMolecular Microbiology, 2004
- Presence of Active Aliphatic Amidases in Helicobacter Species Able To Colonize the StomachInfection and Immunity, 2003
- Detailed Structural Analysis of the Peptidoglycan of the Human Pathogen Neisseria meningitidisPublished by Elsevier BV ,2003
- Characterization of Staphylococcus aureus Cell Wall Glycan Strands, Evidence for a New β-N-Acetylglucosaminidase ActivityPublished by Elsevier BV ,2000
- Characterization of three different lytic transglycosylases inEscherichia coliFEMS Microbiology Letters, 1993
- Cloning of Campylobacter jejuni genes required for leucine biosynthesis, and construction of leu-negative mutant of C. jejuni by shuttle transposon mutagenesisResearch in Microbiology, 1992
- Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresisJournal of Immunological Methods, 1990
- Exoenzymatic Activity of Transglycosylase Isolated from Escherichia coliEuropean Journal of Biochemistry, 1981