The Proliferation and Differentiation of Neonatal Epidermal Melanocytes in F1Hairless Mice of HR-1 × HR/De in Serum-Free Culture

Abstract

To investigate the characteristics of the proliferation and differentiation of epidermal melanocytes in F₁ hairless mice of HR‐1 × HR/De parents in vitro, cell suspensions of the neonatal epidermis were cultured in a serum‐free medium supplemented with dibutyryl adenosine 3‘,5‘‐cyclic monophosphate (DBcAMP) and/or basic fibroblast growth factor (bFGF). The differentiation of melanocytes was induced by treatment with DBcAMP. In contrast, the sustained proliferation of melanoblasts was induced by combined treatment with DBcAMP and bFGF. The melanoblasts could be subcultured in serum‐free medium supplemented with the two factors in the presence of keratinocytes, but not in their absence. This is the first report of successful culture of melanoblasts and melanocytes from hairless mice; it is expected to be useful in understanding the mechanism of the development of pigmented spots in the epidermis of (HR‐1 × HR/De) F₁ mice, which are reported to be induced by repeated exposure to ultraviolet light B.