[34] Mutagenesis with degenerate oligonucleotides: An efficient method for saturating a defined DNA region with base pair substitutions
- 1 January 1987
- book chapter
- Published by Elsevier BV in Methods in enzymology
- Vol. 155, 558-568
- https://doi.org/10.1016/0076-6879(87)55036-4
Abstract
No abstract availableThis publication has 11 references indexed in Scilit:
- Cloning of random-sequence oligodeoxynucleotidesGene, 1986
- A simple and efficient procedure for saturation mutagenesis using mixed oligodeoxynucleotidesGene, 1986
- Saccharomyces cerevisiae CYC1 mRNA 5'-end positioning: analysis by in vitro mutagenesis, using synthetic duplexes with random mismatch base pairs.Molecular and Cellular Biology, 1985
- Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNADNA, 1985
- Analytical studies of ‘mixed sequence’ oligodeoxyribonucleotides synthesized by competitive coupling of either methyl- or β-cyanoethyl-N,N-diisopropylamino phosphoramidite reagents, including 2′-deoxyinosineNucleic Acids Research, 1985
- Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5‘ of an ATG initiation codonNucleic Acids Research, 1983
- Regulatory sites for his3 gene expression in yeastNature, 1982
- Synthesis of deoxyoligonucleotides on a polymer supportJournal of the American Chemical Society, 1981
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesisTetrahedron Letters, 1981
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980