Purification and characterization of the double-stranded DNA-activated protein kinase, DNA-PK, from human placenta
- 1 January 1996
- journal article
- Published by Canadian Science Publishing in Biochemistry and Cell Biology
- Vol. 74 (1), 67-73
- https://doi.org/10.1139/o96-007
Abstract
The double-stranded DNA-activated protein kinase (DNA-PK) is a serine–threonine protein kinase that is composed of a large catalytic subunit (p350) and a DNA-binding protein of 70 and 80 kDa subunits known as the Ku autoantigen. When targeted to DNA by free DNA ends, DNA-PK phosphorylates many DNA- binding proteins and transcription factors. Previously, DNA-PK had only been purified and characterized from transformed human tissue culture cells. Here we report that DNA-PK is an abundant protein in human placenta and lymphocytes. We have purified the placental DNA-PK to homogeneity and show that its biochemical properties are similar to those of the HeLa cell enzyme.Key words: DNA-activated protein kinase.Keywords
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