Messenger RNA activities of four acute phase proteins during inflammation

Abstract

Poly(A)⁺ RNA isolated from the livers of normal rats and of rats suffering from an acute inflammation was translated in a cell-free translation system from rabbit reticulocytes. The translation products were immunoprecipitated with specific antisera against α1-acid glycoprotein, α2-macroglobulin, transferrin, α1-proteinase inhibitor and albumin. 15 to 21 h after intramuscular injection of turpentine 73-, 66-, 2.8-, and 2-fold increases in translatable mRNAs for α1-acid glycoprotein, α2-macroglobulin, transferrin and α1-proteinase inhibitor, respectively, were observed. For albumin a decrease in translatable mRNA to about 30% of controls was measured.