The impact of multiple splice sites in human L1 elements
- 11 January 2008
- journal article
- Published by Elsevier BV in Gene
- Vol. 411 (1-2), 38-45
- https://doi.org/10.1016/j.gene.2007.12.022
Abstract
LINE-1 elements represent a significant proportion of mammalian genomes. The impact of their activity on the structure and function of the host genomes has been recognized from the time of their discovery as an endogenous source of insertional mutagenesis. L1 elements contain numerous functional internal polyadenylation signals and splice sites that generate a variety of processed L1 transcripts. These sites are also reported to contribute to the generation of hybrid transcripts between L1 elements and host genes. Using northern blot analysis we demonstrate that L1 splicing, but not L1 polyadenylation, is delayed during the course of L1 expression. L1 splicing can also be negatively regulated by EBV SM protein known to alter this process. These results suggest a potential for L1 mRNA processing to be regulated in a tissue- and/or development-specific manner. The delay in L1 splicing may also serve to protect host genes from the excessive burden of L1 interference with their normal expression via aberrant splicing.Keywords
This publication has 51 references indexed in Scilit:
- Differing patterns of selection in alternative and constitutive splice sitesGenome Research, 2007
- Widespread mRNA polyadenylation events in introns indicate dynamic interplay between polyadenylation and splicingGenome Research, 2007
- Cellular inhibitors of long interspersed element 1 and Alu retrotranspositionProceedings of the National Academy of Sciences of the United States of America, 2006
- The Human LINE-1 Retrotransposon Creates DNA Double-strand BreaksJournal of Molecular Biology, 2006
- L1 Antisense Promoter Drives Tissue-Specific Transcription of Human GenesJournal of Biomedicine and Biotechnology, 2006
- Nickel Stimulates L1 Retrotransposition by a Post-transcriptional MechanismJournal of Molecular Biology, 2005
- LINE-mediated retrotransposition of marked Alu sequencesNature Genetics, 2003
- Initial sequencing and comparative analysis of the mouse genomeNature, 2002
- DNA methylation in cancer: too much, but also too littleOncogene, 2002
- Initial sequencing and analysis of the human genomeNature, 2001