Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development
Open Access
- 31 October 2017
- journal article
- review article
- Published by MDPI AG in Biosensors
- Vol. 7 (4), 49
- https://doi.org/10.3390/bios7040049
Abstract
Bio-layer interferometry (BLI) real-time, label-free technology has greatly contributed to advances in vaccine research and development. BLI Octet platforms offer high-throughput, ease of use, reliability, and high precision analysis when compared with common labeling techniques. Many different strategies have been used to immobilize the pathogen or host molecules on BLI biosensors for real-time kinetics and affinity analysis, quantification, or high-throughput titer. These strategies can be used in multiple applications and shed light onto the structural and functional aspects molecules play during pathogen-host interactions. They also provide crucial information on how to achieve protection. This review summarizes some key BLI strategies used in human vaccine research and development.Keywords
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